Artículo
Reversible exposure of hydrophobic residues on albumin as a novel strategy for formulation of nanodelivery vehicles for taxanes
Garro, Ariel Gustavo; Beltramo, Dante Miguel
; Alasino, Roxana Valeria
; Leonhard, Victoria
; Heredia, Valeria; Bianco, Ismael Dario
Fecha de publicación:
06/2011
Editorial:
Dove Press
Revista:
International Journal of Nanomedicine
ISSN:
1176-9114
e-ISSN:
1178-2013
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We report herein a novel strategy for the preparation of protein-based nanodelivery vehicles for hydrophobic active pharmaceutical ingredients. The procedure consisted of three steps, ie, exposure of hydrophobic residues of a protein to a pH-induced partial unfolding: interaction between hydrophobic residues on the protein and the hydrophobic active pharmaceutical ingredient, and a final step where the structure of the protein was reversed to a native-like state by returning to neutral pH. As proof of concept, the interaction of paclitaxel with partially unfolded states of human serum albumin was evaluated as a potential method for the preparation of water-soluble complexes of the taxane with albumin. We found that paclitaxel readily binds to pH-induced partially unfolded albumin, leading to the formation of optically clear water-soluble complexes. The complexes thus formed were more stable in solution when the albumin native state was at least partially restored by neutralization of the solution to a pH around 7. It was also observed that the hydrodynamic radius of human serum albumin was only slightly increased after the cycle of pH changes, remaining in a monomeric state with a size according to paclitaxel binding. Furthermore, paclitaxel binding did not affect the overall exposure of charged groups of human serum albumin, as evaluated by its interaction with an ionic exchange resin. The in vitro biological activity of the complexes formed was qualitatively equivalent to that of a Cremophor®-based formulation.
Palabras clave:
HUMAN SERUM ALBUMIN
,
PACLITAXEL
,
UNFOLDED STATES
,
SOLUBILITY
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Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Garro, Ariel Gustavo; Beltramo, Dante Miguel; Alasino, Roxana Valeria; Leonhard, Victoria; Heredia, Valeria; et al.; Reversible exposure of hydrophobic residues on albumin as a novel strategy for formulation of nanodelivery vehicles for taxanes; Dove Press; International Journal of Nanomedicine; 6; 6-2011; 1193-1200
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