Artículo
Cytogenetic markers for the characterization of Capsicum annuum L. cultivars
Romero, María Victoria
; Urdampilleta, Juan Domingo
; Martins, Eliana Martina Forni; Moscone, Eduardo Alberto
Fecha de publicación:
03/2016
Editorial:
Taylor & Francis Ltd
Revista:
Plant Biosystems
ISSN:
1126-3504
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Karyotypic studies with conventional staining have been unsuccessful due to the uniformity of Capsicum chromosomes. In this study, we found diagnostic chromosome characters that permit to characterize cultivars; this is the first cytological characterization of both rDNAs (18S and 5S) in a species of Capsicum using a genus specific probe and the most exhaustive in C. annuum to date. The heterochromatic banding patterns enabled us to identify cultivars, and fluorescent in situ hybridization (FISH) showed one 5S rDNA locus largely conserved within the cultivars, whereas high variation in the number of 18S rDNA loci was observed. One of the most obvious differences is the presence of an additional active nucleolar organizer region in pair #12 and the dispersal of inactive 18S rDNA signals. These results indicate that fluorochrome banding together with silver impregnation and FISH procedures are very useful for the identification of chromosomes and the interpretation of chromosomal variation between cultivars. The functional role of this variation is still uncertain, but our results show that copy number variation of repetitive DNA during the course of evolution might provide an excellent experimental system for studying genome rearrangements accompanying functional divergence in domesticated C. annuum.
Palabras clave:
Capsicum Annuum
,
Ribosomal Dna
,
Fish
,
Fluorochrome Banding
,
Silver Staining
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IMBIV)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Citación
Romero, María Victoria; Urdampilleta, Juan Domingo; Martins, Eliana Martina Forni ; Moscone, Eduardo Alberto; Cytogenetic markers for the characterization of Capsicum annuum L. cultivars; Taylor & Francis Ltd; Plant Biosystems; 151; 1; 3-2016; 84-91
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