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dc.contributor.author
Calgua, B.
dc.contributor.author
Fumian, T.
dc.contributor.author
Rusinol, M.
dc.contributor.author
Rodríguez Manzano, J.
dc.contributor.author
Mbayed, Viviana Andrea
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dc.contributor.author
Bofill Mas, S.
dc.contributor.author
Miagostovich, M.
dc.contributor.author
Girones, R.
dc.date.available
2015-09-03T18:19:25Z
dc.date.issued
2013-03-15
dc.identifier.citation
Calgua, B.; Fumian, T.; Rusinol, M.; Rodríguez Manzano, J.; Mbayed, Viviana Andrea; et al.; Detection and quantification of classic and emerging viruses by skimmed-milk flocculation and PCR in river water from two geographical areas; Elsevier; Water Research; 47; 8; 15-3-2013; 2797-2810
dc.identifier.issn
0043-1354
dc.identifier.uri
http://hdl.handle.net/11336/1925
dc.description.abstract
Molecular techniques and virus concentration methods have shown that previously unknown viruses are shed by humans and animals, and may be transmitted by sewage-contaminated water. In the present study, river water from urban areas in Barcelona, Spain and Rio de Janeiro, Brazil, were analyzed to evaluate the dissemination of human viruses, while simultaneously optimizing and validating a low-cost concentration method for virus quantification in fresh water. The following three viral groups were analyzed. (i) Recently described viruses: klassevirus (KV), asfarvirus-like virus (ASFLV), and the polyomaviruses Merkel cell, KI and WU (MCPyV/KIPyV/WUPyV). (ii) Gastroenteritis agents: noroviruses (NoV) and rotaviruses (RV). (iii) Human fecal viral indicators in water: human adenoviruses (HAdV) and JC polyomaviruses (JCPyV). Virus detection was based on nested and quantitative PCR assays. Nested PCR assays were developed for KV and ASFLV. The method applied for virus concentration in water samples was a one-step procedure based on a skimmed milk flocculation procedure described previously for seawater. Using spiked river water samples, inter- and intra-laboratory assays showed a viral recovery rate of about 50% for HAdV, JCPyV, NoV and RV with a coefficient of variation ≤ 50%. HAdV and JCPyV were detected in 100% of the river samples from Barcelona and Rio de Janeiro. Moreover, NoV GGII was detected in 100% and MCPyV in 50% of the samples from Barcelona, whereas none of the other viruses analyzed were detected. NoV GGII was detected in 33%, KV in 33%, ASFLV in 17% and MCPyV in 50% of the samples from Rio de Janeiro, whereas KIPyV and WUPyV were not detected. RV were only tested for in Rio de Janeiro and resulted positive in 67% of the samples. The procedure applied here to river water represents a useful, straightforward and cost-effective method that could be applied in routine water quality testing. The results of the assays expand our understanding of the global distribution of the viral pathogens studied here and their persistence in the environment.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Emerging Viruses
dc.subject
Human Polyomaviruses
dc.subject
Merkel Cell Polyomavirus
dc.subject
Klassevirus
dc.subject.classification
Oceanografía, Hidrología, Recursos Hídricos
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dc.subject.classification
Ciencias de la Tierra y relacionadas con el Medio Ambiente
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.subject.classification
Virología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Detection and quantification of classic and emerging viruses by skimmed-milk flocculation and PCR in river water from two geographical areas
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
47
dc.journal.number
8
dc.journal.pagination
2797-2810
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Calgua, B.. Universidad de Barcelona; España;
dc.description.fil
Fil: Fumian, T.. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz; Brasil;
dc.description.fil
Fil: Rusinol, M.. Universidad de Barcelona; España;
dc.description.fil
Fil: Rodríguez Manzano, J.. Universidad de Barcelona; España;
dc.description.fil
Fil: Mbayed, Viviana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
dc.description.fil
Fil: Bofill Mas, S.. Universidad de Barcelona; España;
dc.description.fil
Fil: Miagostovich, M.. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz; Brasil;
dc.description.fil
Fil: Girones, R.. Universidad de Barcelona; España;
dc.journal.title
Water Research
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0043135413001474
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/doi:10.1016/j.watres.2013.02.043
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