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dc.contributor.author Galera, Ivana Laura Delia
dc.contributor.author Paraje, Maria Gabriela
dc.contributor.author Paez, Paulina Laura
dc.date.available 2017-06-30T14:16:25Z
dc.date.issued 2016-02-18
dc.identifier.citation Galera, Ivana Laura Delia; Paraje, Maria Gabriela; Paez, Paulina Laura; Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria; International Knowledge Press; Journal of Biology and Nature; 5; 3; 18-2-2016; 2395-5376
dc.identifier.issn 2395-5376
dc.identifier.uri http://hdl.handle.net/11336/19246
dc.description.abstract Oxygen is essential to the life of the aerobic organisms, and most is used for the intracellular generation of energy, where several molecules are formed by partial reduction of oxygen. Because of its short half-life, low selectivity and high reactivity, biological targets of reactive oxygen species (ROS) include various components such as proteins, nucleic acids and lipids. The purpose of this work was to establish a relationship between ROS and reactive nitrogen intermediates (RNI) generation in bacteria exposed to antibiotics. ROS was determined by the reduction of nitro blue tetrazolium (NBT). In aqueous solutions, nitric oxide is converted to nitrite (NO2-), this was determined using a non-enzymatic colorimetric assay. NBT studies indicated stimulation of oxidative stress with an increase of ROS generated by the antibiotic in the bacteria. We observed an increase in the production of NO2- dose-dependent with CIP and GEN. The determination of ROS and RNI allowed establish differences in the alteration of oxidative metabolism in susceptible and resistant strains. Resistance to CIP and GEN may be related to the decreased production of ROS and RNI and consequently, a smaller macromolecules bacterial oxidation. These results contribute to a better understanding of the ROS and RNI regulation.
dc.format application/pdf
dc.language.iso eng
dc.publisher International Knowledge Press
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject OXIDATIVE STRESS
dc.subject BACTERIA
dc.subject NITROSATIVE STRESS
dc.subject ANTIBIOTICS
dc.subject.classification Biología Celular, Microbiología
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-06-29T13:55:35Z
dc.identifier.eissn 2395-5384
dc.journal.volume 5
dc.journal.number 3
dc.journal.pagination 2395-5376
dc.journal.pais India
dc.journal.ciudad India
dc.description.fil Fil: Galera, Ivana Laura Delia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina
dc.description.fil Fil: Paraje, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
dc.description.fil Fil: Paez, Paulina Laura. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Journal of Biology and Nature
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.ikpress.org/abstract/5319


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)