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dc.contributor.author
Leopardo, Noelia Paola  
dc.contributor.author
Jensen, Cristian Federico  
dc.contributor.author
Willis, Miguel Alfredo  
dc.contributor.author
Espinosa, Maria Beatriz  
dc.contributor.author
Vitullo, Alfredo Daniel  
dc.date.available
2023-04-03T11:47:25Z  
dc.date.issued
2011-05  
dc.identifier.citation
Leopardo, Noelia Paola; Jensen, Cristian Federico; Willis, Miguel Alfredo; Espinosa, Maria Beatriz; Vitullo, Alfredo Daniel; The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition; BioScientifica; Reproduction; 141; 5; 5-2011; 633-641  
dc.identifier.issn
1470-1626  
dc.identifier.uri
http://hdl.handle.net/11336/192428  
dc.description.abstract
Apoptosis-dependent massive germ cell death is considered a constitutive trait of the developing mammalian ovary that eliminates 65-85% of the germinal tissue depending on the species. After birth and during adult lifetime, apoptotic activity moves from the germ cell proper to the somatic compartment, decimating germ cells through follicular atresia until the oocyte reserve is exhausted. In contrast, the South American rodent Lagostomus maximus shows suppressed apoptosis-dependent follicular atresia in the adult ovary, with continuous folliculogenesis and massive polyovulation, which finally exhausts the oocyte pool. The absence of follicular atresia in adult L. maximus might arise from a failure to move apoptosis from the germinal stratum to the somatic compartment after birth or being a constitutive trait of the ovarian tissue with no massive germ cell degeneration in the developing ovary. We tested these possibilities by analysing oogenesis, expression of germ cell-specific VASA protein, apoptotic proteins BCL2 and BAX, and DNA fragmentation by TUNEL assay in the developing ovary of L. maximus. Immunolabelling for VASA revealed a massive and widespread colonisation of the ovary and proliferation of germ cells organised in nests that disappeared at late development when folliculogenesis began. No sign of germ cell attrition was found at any time point. BCL2 remained positive throughout oogenesis, whereas BAXwas slightly detected in early development. TUNEL assay was conspicuously negative throughout the development. These results advocate for an unrestricted proliferation of germ cells, without apoptosis-driven elimination, as a constitutive trait of L. maximus ovary as opposed to what is normally found in the developing mammalian ovary.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
BioScientifica  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
LAGOSTOMUS MAXIMUS  
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FETAL OVARY  
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GERM CELL  
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APOPTOSIS  
dc.subject.classification
Biología del Desarrollo  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
The developing ovary of the South American plains vizcacha, Lagostomus maximus (Mammalia, Rodentia): Massive proliferation with no sign of apoptosis-mediated germ cell attrition  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-03-30T14:42:29Z  
dc.journal.volume
141  
dc.journal.number
5  
dc.journal.pagination
633-641  
dc.journal.pais
Reino Unido  
dc.description.fil
Fil: Leopardo, Noelia Paola. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Jensen, Cristian Federico. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Willis, Miguel Alfredo. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Espinosa, Maria Beatriz. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Vitullo, Alfredo Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.journal.title
Reproduction  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/141/5/633.abstract  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1530/REP-10-0463