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dc.contributor.author
Kucinskaite Kodze, Indre
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Petraityte Burneikiene, Rasa
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Zvirbliene, Aurelija
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Hjelle, Brian
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Medina, Rafael A.
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Gedvilaite, Alma
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Razanskiene, Ausra
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Schmidt Chanasit, Jonas
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Mertens, Marc
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Padula, Paula Julieta
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Sasnauskas, Kestutis
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Ulrich, Rainer G.
dc.date.available
2023-03-30T12:30:09Z
dc.date.issued
2011-03
dc.identifier.citation
Kucinskaite Kodze, Indre; Petraityte Burneikiene, Rasa; Zvirbliene, Aurelija; Hjelle, Brian; Medina, Rafael A.; et al.; Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples; Springer Wien; Archives of Virology; 156; 3; 3-2011; 443-456
dc.identifier.issn
0304-8608
dc.identifier.uri
http://hdl.handle.net/11336/192109
dc.description.abstract
Monoclonal antibodies are important tools for various applications in hantavirus diagnostics. Recently, we generated Puumala virus (PUUV)-reactive monoclonal antibodies (mAbs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (VLPs) harbouring the 120-amino-acid-long amino-terminal region of the PUUV nucleocapsid (N) protein. Here, we describe the generation of two mAbs by co-immunisation of mice with hexahistidine-tagged full-length N proteins of Sin Nombre virus (SNV) and Andes virus (ANDV), their characterization by different immunoassays and comparison with the previously generated mAbs raised against a segment of PUUV N protein inserted into VLPs. All of the mAbs reacted strongly in ELISA and western blot tests with the antigens used for immunization and cross-reacted to varying extents with N proteins of other hantaviruses. All mAbs raised against a segment of the PUUV N protein presented on chimeric VLPs and both mAbs raised against the full-length AND/SNV N protein reacted with Vero cells infected with different hantaviruses. The reactivity of mAbs with native viral nucleocapsids was also confirmed by their reactivity in immunohistochemistry assays with kidney tissue specimens from experimentally SNV-infected rodents and human heart tissue specimens from hantavirus cardiopulmonary syndrome patients. Therefore, the described mAbs represent useful tools for the immunodetection of hantavirus infection.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Wien
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Antibodies
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Monoclonal Antibodies
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Viral Nucleocapsid Proteins
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hantavirus
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Virología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-03-28T14:22:14Z
dc.journal.volume
156
dc.journal.number
3
dc.journal.pagination
443-456
dc.journal.pais
Austria
dc.journal.ciudad
Viena
dc.description.fil
Fil: Kucinskaite Kodze, Indre. Vilnius University; Lituania
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Fil: Petraityte Burneikiene, Rasa. Vilnius University; Lituania
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Fil: Zvirbliene, Aurelija. Vilnius University; Lituania
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Fil: Hjelle, Brian. University Of New Mexico. School Of Medicine; Estados Unidos
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Fil: Medina, Rafael A.. University Of New Mexico. School Of Medicine; Estados Unidos
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Fil: Gedvilaite, Alma. Vilnius University; Lituania
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Fil: Razanskiene, Ausra. Vilnius University; Lituania
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Fil: Schmidt Chanasit, Jonas. No especifíca;
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Fil: Mertens, Marc. No especifíca;
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Fil: Padula, Paula Julieta. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Sasnauskas, Kestutis. Vilnius University; Lituania
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Fil: Ulrich, Rainer G.. No especifíca;
dc.journal.title
Archives of Virology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00705-010-0879-6
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00705-010-0879-6
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