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dc.contributor.author
Martin, Anandi
dc.contributor.author
Paasch, Fabienne
dc.contributor.author
Docx, Sven
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Fissette, Krista
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Imperiale, Belén Rocío
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dc.contributor.author
Ribón, Wellman
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González, Liliana Andrea
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Werngren, Jim
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Engström, Anna
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Skenders, Girts
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Juréen, Pontus
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Hoffner, Sven
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Del Portillo, Patricia
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Morcillo, Nora Susana
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dc.contributor.author
Palomino, Juan Carlos
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dc.date.available
2023-03-30T12:27:41Z
dc.date.issued
2011-04
dc.identifier.citation
Martin, Anandi; Paasch, Fabienne; Docx, Sven; Fissette, Krista; Imperiale, Belén Rocío; et al.; Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis; Oxford University Press; Journal of Antimicrobial Chemotherapy; 66; 4; 4-2011; 827-833
dc.identifier.issn
0305-7453
dc.identifier.uri
http://hdl.handle.net/11336/192105
dc.description.abstract
Objectives: To perform a multicentre study to evaluate the performance of the colorimetric redox indicator (CRI) assay and to establish the MICs and critical concentrations of rifampicin, isoniazid, ofloxacin, kanamycin and capreomycin. Methods: The study was carried out in two phases. Phase I determined the MIC of each drug. Phase II established critical concentrations for the five drugs tested by the CRI assay compared with the conventional proportion method. Results: Phase I: a strain was considered resistant by the CRI assay if the MIC was 0.5 mg/L for rifampicin, 0.25 mg/L for isoniazid, 4.0 mg/L for ofloxacin and 5.0 mg/L for kanamycin and capreomycin. Sensitivity was 99.1% for isoniazid and 100% for the other drugs and specificity was 97.9% for capreomycin and 100% for the other drugs. Phase II: the critical concentration was 0.5 mg/L for rifampicin, 0.25 mg/L for isoniazid, 2.0 mg/L for ofloxacin and 2.5 mg/L for kanamycin and capreomycin giving an overall accuracy of 98.4%, 96.6%, 96.7%, 98.3% and 90%, respectively. Conclusions: Results demonstrate that the CRI assay is an accurate method for the rapid detection of XDR Mycobacterium tuberculosis. The CRI assay is faster than the conventional drug susceptibility testing method using solid medium, has the same turnaround time as the BACTEC MGIT 960 system, but is less expensive, and could be an adequate method for low-income countries.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DRUG RESISTANCE
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RESAZURIN
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SECOND-LINE DRUGS
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TUBERCULOSIS
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-03-29T17:21:32Z
dc.journal.volume
66
dc.journal.number
4
dc.journal.pagination
827-833
dc.journal.pais
Reino Unido
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dc.description.fil
Fil: Martin, Anandi. Institute of Tropical Medicine; Bélgica
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Fil: Paasch, Fabienne. Institute of Tropical Medicine; Bélgica
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Fil: Docx, Sven. Institute of Tropical Medicine; Bélgica
dc.description.fil
Fil: Fissette, Krista. Institute of Tropical Medicine; Bélgica
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Fil: Imperiale, Belén Rocío. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Ribón, Wellman. Universidad Industrial Santander; Colombia
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Fil: González, Liliana Andrea. Universidad Industrial Santander; Colombia
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Fil: Werngren, Jim. Swedish Institute for Infectious Disease Control; Suecia
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Fil: Engström, Anna. Swedish Institute for Infectious Disease Control; Suecia
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Fil: Skenders, Girts. No especifíca;
dc.description.fil
Fil: Juréen, Pontus. Swedish Institute for Infectious Disease Control; Suecia
dc.description.fil
Fil: Hoffner, Sven. Swedish Institute for Infectious Disease Control; Suecia
dc.description.fil
Fil: Del Portillo, Patricia. No especifíca;
dc.description.fil
Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
dc.description.fil
Fil: Palomino, Juan Carlos. Institute of Tropical Medicine; Bélgica
dc.journal.title
Journal of Antimicrobial Chemotherapy
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jac/article/66/4/827/723957
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/jac/dkq527
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