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dc.contributor.author
Frías, María de los Ángeles
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dc.contributor.author
Benesch, Matthew G. K.
dc.contributor.author
Lewis, Ruthven N. A. H.
dc.contributor.author
McElhaney, Ronald N.
dc.date.available
2023-03-29T16:09:44Z
dc.date.issued
2011-03
dc.identifier.citation
Frías, María de los Ángeles; Benesch, Matthew G. K.; Lewis, Ruthven N. A. H.; McElhaney, Ronald N.; On the miscibility of cardiolipin with 1,2-diacyl phosphoglycerides: Binary mixtures of dimyristoylphosphatidylethanolamine and tetramyristoylcardiolipin; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1808; 3; 3-2011; 774-783
dc.identifier.issn
0005-2736
dc.identifier.uri
http://hdl.handle.net/11336/192062
dc.description.abstract
The thermotropic phase behavior and organization of model membranes composed of binary mixtures of the quadruple-chained, anionic phospholipid tetramyristoylcardiolipin (TMCL) with the double-chained zwitterionic phospholipid dimyristoylphosphatidylethanolamine (DMPE) were examined by a combination of differential scanning calorimetry (DSC) and Fourier-transform infrared (FTIR) spectroscopy. After equilibration at low temperature, DSC thermograms exhibited by binary mixtures of TMCL and DMPE containing < 80 mol DMPE exhibit a fairly energetic lower temperature endotherm and a highly energetic higher temperature endotherm. As the relative amount of TMCL in the mixture decreases, the temperature, enthalpy and cooperativity of the lower temperature endotherm also decreases and is not calorimetrically detectable when the TMCL content falls below 20 mol%. In contrast, the temperature of the higher temperature endotherm increases as the proportion of TMCL decreases, but the enthalpy and cooperativity both decrease and the transition endotherms become multimodal. The FTIR spectroscopic results indicate that the lower temperature endotherm corresponds to a lamellar crystalline (Lc) to lamellar gel (Lβ) phase transition and that the higher temperature transition involves the conversion of the Lβ phase to the lamellar liquid-crystalline (Lα) phase. Moreover, the FTIR spectroscopic signatures observed at temperatures below the onset of the Lc/Lβ phase transitions are consistent with the coexistence of structures akin to a TMCL-like Lc phase and the L β phase, and with the relative amount of the TMCL-like L c phase increasing progressively as the TMCL content of the mixture increases. These latter observations suggest that the TMCL and DMPE components of these mixtures are poorly miscible at temperatures below the L β/Lα phase transition temperature. Poor miscibility of these two components is also suggested by the complexity of the DSC thermograms observed at the Lβ/Lα phase transitions of these mixtures and with the complex relationship between their Lβ/Lα phase transition temperatures and the composition of the mixture. Overall, our data suggests that TMCL and DMPE may be intrinsically poorly miscible across a broad composition range, notwithstanding the homogeneity of the fatty acid chains of the two components and the modest (~ 10 °C) difference between their Lβ/Lα phase transition temperatures.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
thermotropic phase behavior
dc.subject
binary mixtures
dc.subject
endotherm
dc.subject
homogeneity
dc.subject.classification
Otras Ciencias Naturales y Exactas
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dc.subject.classification
Otras Ciencias Naturales y Exactas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
On the miscibility of cardiolipin with 1,2-diacyl phosphoglycerides: Binary mixtures of dimyristoylphosphatidylethanolamine and tetramyristoylcardiolipin
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-03-28T14:23:33Z
dc.journal.volume
1808
dc.journal.number
3
dc.journal.pagination
774-783
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Frías, María de los Ángeles. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Benesch, Matthew G. K.. University of Alberta; Canadá
dc.description.fil
Fil: Lewis, Ruthven N. A. H.. University of Alberta; Canadá
dc.description.fil
Fil: McElhaney, Ronald N.. University of Alberta; Canadá
dc.journal.title
Biochimica et Biophysica Acta - Biomembranes
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005273610004451
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbamem.2010.12.010
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