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dc.contributor.author
Kemppainen, Minna Johanna
dc.contributor.author
Pardo, Alejandro Guillermo
dc.date.available
2023-03-27T20:08:18Z
dc.date.issued
2011-01
dc.identifier.citation
Kemppainen, Minna Johanna; Pardo, Alejandro Guillermo; Transformation of the mycorrhizal fungus laccaria bicolor by using agrobacterium tumefaciens; Taylor & Francis; Bioengineered Bugs; 2; 1; 1-2011; 38-44
dc.identifier.issn
1949-1018
dc.identifier.uri
http://hdl.handle.net/11336/191742
dc.description.abstract
Most boreal and temperate forest trees form a mutualistic symbiosis with soil borne fungi called ectomycorrhiza (EC M). In this association both partners benefit due to nutrient exchange at the symbiotic interface. Laccaria bicolor is the first mycorrhizal fungus with its genome sequenced thus making possible for the first time to analyze genome scale gene expression profiles of a mutualistic fungus. However, in order to be able to take full advantage of the genome sequence, reverse genetic tools are needed. Among them a high throughput transformation system is crucial. Herein we present a detailed protocol for genetic transformation of Laccaria bicolor by means of Agrobacterium tumefaciens with emphasis on critical steps affecting the success and efficiency of the approach.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AGROBACTERIUM
dc.subject
ECTOMYCORRHIZA
dc.subject
LACCARIA
dc.subject
T-DNA
dc.subject
TRANSFORMATION
dc.subject.classification
Micología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Transformation of the mycorrhizal fungus laccaria bicolor by using agrobacterium tumefaciens
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-03-23T12:31:46Z
dc.journal.volume
2
dc.journal.number
1
dc.journal.pagination
38-44
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Kemppainen, Minna Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
dc.description.fil
Fil: Pardo, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
dc.journal.title
Bioengineered Bugs
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.4161/bbug.2.1.14394
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.4161/bbug.2.1.14394
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