Mostrar el registro sencillo del ítem
dc.contributor.author
Hernández Neuta, Iván
dc.contributor.author
Varela, Andrés
dc.contributor.author
Martin, Anandi
dc.contributor.author
Von Groll, Andrea
dc.contributor.author
Jureen, Pontus
dc.contributor.author
López, Beatriz
dc.contributor.author
Imperiale, Belén Rocío
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Skenders, Girts
dc.contributor.author
Ritacco, Viviana
dc.contributor.author
Hoffner, Sven
dc.contributor.author
Morcillo, Nora Susana
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Palomino, Juan Carlos
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Del Portillo, Patricia
dc.date.available
2023-03-23T11:49:10Z
dc.date.issued
2010-12
dc.identifier.citation
Hernández Neuta, Iván; Varela, Andrés; Martin, Anandi; Von Groll, Andrea; Jureen, Pontus; et al.; Rifampin-isoniazid oligonucleotide typing: An alternative format for rapid detection of multidrug-resistant Mycobacterium tuberculosis; American Society for Microbiology; Journal of Clinical Microbiology; 48; 12; 12-2010; 4386-4391
dc.identifier.issn
0095-1137
dc.identifier.uri
http://hdl.handle.net/11336/191515
dc.description.abstract
A reverse line blot DNA hybridization format for rapid detection of multidrug-resistant tuberculosis was developed. Simultaneous detection of rifampin and isoniazid resistance in clinical isolates of Mycobacterium tuberculosis was based on the same amplification/reverse hybridization principle of the widely used spoligotyping. The test involved probing nine DNA regions that are targets of common drug resistance-associated mutations in the genes rpoB, katG, and inhA. Addition of quaternary amine tetramethyl ammonium chloride to the hybridization buffer promoted multiple hybrid formations at a single annealing temperature irrespective of the different GC contents of probes. The assay was standardized using 20 well-documented strains from the Institute of Tropical Medicine (Belgium) and evaluated blindly in a central laboratory with 100 DNA samples that were obtained from cultured clinical isolates and shipped dried from three other countries. Compared with drug susceptibility testing, both sensitivity and specificity for rifampin resistance detection were 93.0% while for isoniazid the values were 87.7% and 97.7%, respectively. Compared with sequencing and GenoType MTBDRplus methods, sensitivity and specificity reached 96.4% and 95.5% for rifampin and 92.7% and 100% for isoniazid. Altogether, 40/45 (89%) multidrug-resistant isolates were correctly identified. Advantages of this in-house development include versatility, capacity to run up to 41 samples by triplicate in a single run, and reuse of the membrane at least 10 times. These features substantially reduce cost per reaction and make the assay an attractive tool for use in reference laboratories of countries that have a high burden of multidrugresistant tuberculosis but that cannot afford expensive commercial tests because of limited resources.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
RAPID
dc.subject
MDR-TB
dc.subject
MOLECULAR
dc.subject
DETECTION
dc.subject.classification
Tecnologías que involucran la identificación de ADN, proteínas y enzimas, y cómo influyen en el conjunto de enfermedades y mantenimiento del bienestar
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Biotecnología de la Salud
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Rifampin-isoniazid oligonucleotide typing: An alternative format for rapid detection of multidrug-resistant Mycobacterium tuberculosis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-03-21T18:20:33Z
dc.journal.volume
48
dc.journal.number
12
dc.journal.pagination
4386-4391
dc.journal.pais
Estados Unidos
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.description.fil
Fil: Hernández Neuta, Iván. No especifíca;
dc.description.fil
Fil: Varela, Andrés. No especifíca;
dc.description.fil
Fil: Martin, Anandi. No especifíca;
dc.description.fil
Fil: Von Groll, Andrea. No especifíca;
dc.description.fil
Fil: Jureen, Pontus. No especifíca;
dc.description.fil
Fil: López, Beatriz. No especifíca;
dc.description.fil
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Skenders, Girts. No especifíca;
dc.description.fil
Fil: Ritacco, Viviana. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina
dc.description.fil
Fil: Hoffner, Sven. No especifíca;
dc.description.fil
Fil: Morcillo, Nora Susana. No especifíca;
dc.description.fil
Fil: Palomino, Juan Carlos. No especifíca;
dc.description.fil
Fil: Del Portillo, Patricia. No especifíca;
dc.journal.title
Journal of Clinical Microbiology
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/JCM.00448-10
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/JCM.00448-10
Archivos asociados