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dc.contributor.author
Sauka, Diego Herman
dc.contributor.author
Basurto Ríos, Regina E.
dc.contributor.author
Ibarrrra, Jorge E.
dc.contributor.author
Benintende, Graciela Beatriz
dc.date.available
2023-03-22T18:19:53Z
dc.date.issued
2010-10
dc.identifier.citation
Sauka, Diego Herman; Basurto Ríos, Regina E.; Ibarrrra, Jorge E.; Benintende, Graciela Beatriz; Characterization of an Argentine isolate of Bacillus thuringiensis similar to the HD-1 strain; Sociedade Entomológica do Brasil; Neotropical Entomology; 39; 5; 10-2010; 767-773
dc.identifier.issn
1519-566X
dc.identifier.uri
http://hdl.handle.net/11336/191456
dc.description.abstract
We report the characterization of an Argentine isolate of Bacillus thuringiensis (INTATA24-6) similar to the HD-1 strain, which harbors a cryptic cry2Ab gene that apparently is transcribed but not translated into a protein. INTATA24-6 showed a Rep-PCRpattern identical to the HD-1 strain, a plasmid pattern that resembled that of this strain and cry1 and cry2 genes as HD-1. Screening of cry1 and cry2 genes showed that INTATA24-6 harbors only cry1Ac and cry2Ab genes. Furthermore, crystalline inclusions of INTATA24-6 exhibit a bipyramidal shape, typical of Lepidoptera-active B. thuringiensis strains, containing a major protein of ca. 130 kDa toxic to Epinotia aporema Wals. (Lepidoptera: Tortricidae) larvae. Neither the flat-square to cuboidal crystal nor a ca. 65 kDa protein typical of strains expressing Cry2 proteins were detected in INTATA24-6. In agreement with this information, parasporal crystals of INTATA24-6 did not show toxicity to Aedes aegypti L. (Diptera: Culicidae) larvae. Gene transcription analyses suggested that the cry2A gene might be cryptic in INTATA24-6 despite its transcription at different sporulation stages.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sociedade Entomológica do Brasil
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
CRY1AC
dc.subject
CRY2AB
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CRYPTIC GENE
dc.subject
EPINOTIA APOREMA
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Characterization of an Argentine isolate of Bacillus thuringiensis similar to the HD-1 strain
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-03-15T10:08:51Z
dc.identifier.eissn
1678-8052
dc.journal.volume
39
dc.journal.number
5
dc.journal.pagination
767-773
dc.journal.pais
Brasil
dc.journal.ciudad
Londrina
dc.description.fil
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Microbiologia y Zoologia Agrigola Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Microbiologia y Zoologia Agrigola Al Iabimo.; Argentina
dc.description.fil
Fil: Basurto Ríos, Regina E.. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; México
dc.description.fil
Fil: Ibarrrra, Jorge E.. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; México
dc.description.fil
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
dc.journal.title
Neotropical Entomology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/ne/a/VHWTssxhDpKkFCyLvDNV6Tx/?lang=envvvvvvvv
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1590/S1519-566X2010000500016
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