Artículo
Different primer sets used to amplify by PCR the Hepatitis B virus (HBV) S gene, select different HBV variants
Fecha de publicación:
10/2012
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Journal Of Viral Hepatitis.
ISSN:
1352-0504
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Summary. PCR detection of viral genomes has provided new insights into viral diagnosis. Nowadays, it is the most frequently used nucleic acid testing (qualitative and quantitative) technique. The aim of this study was to analyse the major circulating hepatitis B virus (HBV) variants PCR-amplified by three sets of primers in a patient infected with genotype E. The HBV S/Pol overlapping genomic region was amplified from the serum of an infected child using three primer sets previously described. Sequence analysis corresponding to the HBV S/Pol region revealed the presence of different viral populations depending on the set of primers used. D144A S-escape mutant was detected with two of the primer sets, while the rtL217R mutant within the Pol – conferring resistance to Adefovir – could be picked up with a different pair of primer sets. This study undoubtedly implies that the description of viral polymorphisms should be stated together with the sequence of the primers used for PCR amplification when studies of escape and/or antiviral-resistant HBV mutants are carried out.
Palabras clave:
HEPATITIS B VIRUS
,
PRIMER SETS
,
VARIANTS
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Identificadores
Colecciones
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Cuestas, María Luján; Mathet, Veronica Lidia; Oubiña, Jose Raul; Different primer sets used to amplify by PCR the Hepatitis B virus (HBV) S gene, select different HBV variants; Wiley Blackwell Publishing, Inc; Journal Of Viral Hepatitis.; 19; 10; 10-2012; 754-756
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