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dc.contributor.author
Rapacioli, Melina
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Botelho, Joao
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Cerda, Gustavo
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Duarte, Santiago Pablo
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Elliot, Matías
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Palma, Verónica
dc.contributor.author
Flores, Domingo Vladimir
dc.date.available
2023-03-21T10:50:30Z
dc.date.issued
2012-10
dc.identifier.citation
Rapacioli, Melina; Botelho, Joao; Cerda, Gustavo; Duarte, Santiago Pablo; Elliot, Matías; et al.; Sonic hedgehog (Shh)/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectum; BioMed Central; Bmc Neuroscience; 13; 1; 10-2012; 1-40
dc.identifier.issn
1471-2202
dc.identifier.uri
http://hdl.handle.net/11336/191120
dc.description.abstract
Background: Sonic hedgehog (Shh)/Gli pathway plays an important regulatory role on the neuroepithelial cells (NEc) proliferation in the dorsal regions of the developing vertebrate Central Nervous System. The aim of this paper was to analyze the effect of the Shh/Gli signaling pathway activation on the proliferation dynamics and/or the spatial organization of the NEc proliferation activity during early stages of the developing chick optic tectum (OT). In ovo pharmacological gain and loss of hedgehog function approaches were complemented with in vivo electroporation experiments in order to create ectopic sources of either Shh or Gli activator (GliA) proteins in the OT. NEc proliferating activity was analyzed at ED 4/4.5 by recording the spatial co-ordinates of the entire population of mitotic NEc (mNEc) located along OT dorsal-ventral sections. Several space signals (numerical sequences) were derived from the mNEc spatial co-ordinate records and analyzed by different standardized non-linear methods of signal analysis.Results: In ovo pharmacologic treatment with cyclopamine resulted in dramatic failure in the OT expansion while the agonist purmorphamine produced the opposite result, a huge expansion of the OT vesicle. Besides, GliA and Shh misexpressions interfere with the formation of the intertectal fissure located along the dorsal midline. This morphogenetic alteration is accompanied by an increase in the mNEc density. There is a gradient in the response of NEcs to Shh and GliA: the increase in mNEc density is maximal near the dorsal regions and decrease towards the OT-tegmental boundary. Biomathematical analyses of the signals derived from the mNEc records show that both Shh and GliA electroporations change the proliferation dynamics and the spatial organization of the mNEc as revealed by the changes in the scaling index estimated by these methods.Conclusions: The present results show that the Shh/Gli signaling pathway plays a critical role in the OT expansion and modelling. This effect is probably mediated by a differential mitogenic effect that increases the NEc proliferation and modulates the spatial organization of the NEc proliferation activity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
IN OVO CYCLOPAMINE TREATMENT
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NEUROEPITHELIAL CELL PROLIFERATION
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NON-LINEAR SIGNAL ANALYSES
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OPTIC TECTUM
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PURMORPHAMINE
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SHH/GLI PATHWAY
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Biología del Desarrollo
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Biología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Sonic hedgehog (Shh)/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectum
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-03-20T14:49:08Z
dc.journal.volume
13
dc.journal.number
1
dc.journal.pagination
1-40
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Rapacioli, Melina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro; Argentina
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Fil: Botelho, Joao. Universidad de Chile; Chile
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Fil: Cerda, Gustavo. Universidad de Chile; Chile
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Fil: Duarte, Santiago Pablo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Elliot, Matías. Universidad de Chile; Chile
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Fil: Palma, Verónica. Universidad de Chile; Chile
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Fil: Flores, Domingo Vladimir. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro; Argentina
dc.journal.title
Bmc Neuroscience
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.biomedcentral.com/1471-2202/13/117/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/1471-2202-13-117
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