Artículo
Tubulin pools in human erythrocytes: Altered distribution in hypertensive patients affects Na +, K +-ATPase activity
Amaiden, Marina Rafaela
; Santander, Verónica Silvina
; Monesterolo, Noelia Edith
; Campetelli, Alexis Nazareno
; Rivelli Antonelli, Juan Franco
; Previtali, Gabriela; Arce, Carlos Angel
; Casale, Cesar Horacio
Fecha de publicación:
05/2011
Editorial:
Birkhauser Verlag Ag
Revista:
Cellular and Molecular Life Sciences
ISSN:
1420-682X
e-ISSN:
1420-9071
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The presence of tubulin in human erythrocytes was demonstrated using five different antibodies. Tubulin was distributed among three operationally distinguishable pools: membrane, sedimentable structure and soluble fraction. It is known that in erythrocytes from hypertensive subjects (HS), the Na +, K +-ATPase (NKA) activity is partially inhibited as compared with erythrocytes from normal subjects (NS). In erythrocytes from HS the membrane tubulin pool is increased by ∼150%. NKA was found to be forming a complex with acetylated tubulin that results in inhibition of enzymes. This complex was also increased in erythrocytes from HS. Treatment of erythrocytes from HS with nocodazol caused a decrease of acetylated tubulin in the membrane and stimulation of NKA activity, whereas taxol treatment on erythrocytes from NS had the opposite effect. These results suggest that, in erythrocytes from HS, tubulin was translocated to the membrane, where it associated with NKA with the consequent enzyme inhibition. © Springer Basel AG 2010.
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Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Amaiden, Marina Rafaela; Santander, Verónica Silvina; Monesterolo, Noelia Edith; Campetelli, Alexis Nazareno; Rivelli Antonelli, Juan Franco; et al.; Tubulin pools in human erythrocytes: Altered distribution in hypertensive patients affects Na +, K +-ATPase activity; Birkhauser Verlag Ag; Cellular and Molecular Life Sciences; 68; 10; 5-2011; 1755-1768
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