Mostrar el registro sencillo del ítem
dc.contributor.author
Morcillo, N.
dc.contributor.author
Imperiale, Belén Rocío
dc.contributor.author
Di Giulio, B.
dc.date.available
2023-03-17T14:37:32Z
dc.date.issued
2010-09
dc.identifier.citation
Morcillo, N.; Imperiale, Belén Rocío; Di Giulio, B.; Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 14; 9; 9-2010; 1169-1175
dc.identifier.issn
1027-3719
dc.identifier.uri
http://hdl.handle.net/11336/190897
dc.description.abstract
SETTING: Dr Cetrángolo Hospital, Buenos Aires Province, Argentina. OBJECTIVE: Evaluation of the BACTEC? Mycobacteria Growth Indicator Tube (MGIT)? 960 system and the colorimetric-based method (CMM) for fi rst- and second line drug susceptibility testing (FL-DST, SL-DST) against Mycobacterium tuberculosis. DESIGN: FL-DST was studied using SIRE MGIT 960. Minimal inhibitory concentrations (MICs) for isoniazid (INH), streptomycin, rifampicin (RMP), ethambutol (EMB) and levofl oxacin (LVX) were also determined by CMM using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT). MICs for amikacin (AMK), kanamycin (KM), capreomycin (CPM), ethionamide (ETH), cycloserine, ofl oxacin (OFX), linezolide (LZ) and moxifl oxacin (MFX) were determined on 94 multidrug resistant M. tuberculosis isolates by MGIT 960 and CMM. Statistical methods were applied to define drug susceptible and drug-resistant isolates on the basis of the comparison between results obtained by gold standards. RESULTS: A total of 1626 clinical isolates were studied. Critical drug concentrations could be defi ned in less than 10 days for both CMM and MGIT 960. CMM was cheaper but more laborious than MGIT 960. The highest performances of both methods were achieved for AMK, RMP, OFX, LZ and MFX, followed by INH, ETH, KM, CPM and LVX (tested only by CMM). CONCLUSIONS: Both methods could be implemented as rapid diagnostic tools to detect drug-resistant isolates in clinical practice.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
International Union Against Tuberculosis and Lung Disease
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
TUBERCULOSIS
dc.subject
DRUG- SUSCEPTIBILITY
dc.subject
MGIT960
dc.subject
COLORIMETRIC METHODS
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-03-12T15:42:35Z
dc.journal.volume
14
dc.journal.number
9
dc.journal.pagination
1169-1175
dc.journal.pais
Francia
dc.description.fil
Fil: Morcillo, N.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
dc.description.fil
Fil: Imperiale, Belén Rocío. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Di Giulio, B.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
dc.journal.title
International Journal of Tuberculosis and Lung Disease
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/iuatld/ijtld/2010/00000014/00000009/art00017;jsessionid=6m97djap025ii.x-ic-live-02
Archivos asociados