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dc.contributor.author
Bernal, Anahi Romina
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Polito, Franco S.
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Cruz, Elías L.
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Figueroa, L. I. C
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Fernandez, Pablo Marcelo
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Nieto Peñalver, Carlos Gabriel
dc.date.available
2023-03-14T12:52:12Z
dc.date.issued
2018
dc.identifier.citation
Oxidative stress response of native yeasts to heavy metals; XX Jornadas Anuales de la Sociedad Argentina de Biología y XVII Jornadas de la Sociedad Uruguaya de Biociencias; Ciudad Autónoma de Buenos Aires; Argentina; 2018; 1-2
dc.identifier.uri
http://hdl.handle.net/11336/190447
dc.description.abstract
A possible consequence of heavy metal exposure is an increased production of reactive oxygen species (ROS) that could induce or exacerbate intracellular oxidative stress and can cause physiological stress. Defense mechanisms which counteract the impact of ROS, including enzyme and non-enzyme antioxidant systems, are found in all aerobic cells. In this work we study the antioxidant response of native yeasts to the oxidative stress produced by heavy metals. Yeast strains Meyerozyma guilliermondii 6N and Rhodotorula mucilaginosa 7Apo1 were cultured in YM media in absence/presence of K2Cr2O7 and CuSO4 alone or in metal mixtures. Cell-free extract was prepared and antioxidant enzymatic activities [catalase (CAT), superoxide dismutase (SOD) and thioredoxin reductase (TrxR)] were determined. The specific activity is given as U/mg protein. Total antioxidant capacity was determined by the modified ABTS* discoloration method [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)]. In both strains, the activity of SOD was higher in the presence of Cr (VI) showing significant differences with the control condition without the metal (p < 0.05, Tukey test), the CAT activity, in both strains the highest values were obtained with Cr (VI) and the mixture of metals but this activity was while this activity was reduced in the presence of Cu (II) and the mixture of metals. For lower in the presence of Cu (II). In the case of the TrxR activity, 7Apo1 and 6N did not show significant differences in the assayed conditions (p > 0.05, Tukey test). The total antioxidant capacity of 6N with Cr (VI) and Cu (II) was higher than the control while no differences were observed in the mixture of metals. 7 Apo1 did not show significant differences in the tested conditions (p > 0.05, Tukey test). Interaction between chemical elements, the level of oxidative stress and antioxidant defense play an important role in ecotoxicological response of microorganisms in polluted environments and our results provide additional confirmation for metal-mediated activation of the antioxidant enzymes in yeasts.
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application/pdf
dc.language.iso
eng
dc.publisher
Sociedad Argentina de Biología
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
REACTIVE OXYGEN SPECIES
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OXIDATIVE STRESS
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ANTIOXIDANT ENZYMATIC ACTIVITIES
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YEAST
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Bioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental
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Biotecnología del Medio Ambiente
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Oxidative stress response of native yeasts to heavy metals
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info:eu-repo/semantics/publishedVersion
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info:eu-repo/semantics/conferenceObject
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info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-03-07T12:32:44Z
dc.journal.pagination
1-2
dc.journal.pais
Argentina
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Capital federal
dc.description.fil
Fil: Bernal, Anahi Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Polito, Franco S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Cruz, Elías L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Figueroa, L. I. C. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Fernandez, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
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Autor
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Autor
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Autor
dc.coverage
Internacional
dc.type.subtype
Jornada
dc.description.nombreEvento
XX Jornadas Anuales de la Sociedad Argentina de Biología y XVII Jornadas de la Sociedad Uruguaya de Biociencias
dc.date.evento
2018-12-05
dc.description.ciudadEvento
Ciudad Autónoma de Buenos Aires
dc.description.paisEvento
Argentina
dc.type.publicacion
Book
dc.description.institucionOrganizadora
Sociedad Argentina de Biología
dc.source.libro
XX Jornadas Anuales de la Sociedad Argentina de Biología y XVII Jornadas de la Sociedad Uruguaya de Biociencias
dc.date.eventoHasta
2018-12-07
dc.type
Jornada
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