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dc.contributor.author
Raviolo, Mónica Ana
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dc.contributor.author
Esteve Romero, Josep
dc.contributor.author
Briñon, Margarita Cristina
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dc.date.available
2023-03-10T16:41:30Z
dc.date.issued
2011-05
dc.identifier.citation
Raviolo, Mónica Ana; Esteve Romero, Josep; Briñon, Margarita Cristina; Stability-indicating micellar liquid chromatography method for three novel derivatives of zidovudine in aqueous and simulated gastric and intestinal fluids matrices; Elsevier Science; Journal of Chromatography - A; 1218; 18; 5-2011; 2540-2545
dc.identifier.issn
0021-9673
dc.identifier.uri
http://hdl.handle.net/11336/190223
dc.description.abstract
This work studies the stability of three new anti-HIV agents which were obtained by the association of zidovudine (AZT) with different amino acids, such as leucine (AZT-Leu) and valine (AZT-Val), and one with an acid group (AZT-Ac). Before commercialisation, their stability in different matrices - simulated gastric fluid (SGF, pH 1.2) and simulated intestinal fluid (SIF, pH 6.8), both as the USP 32 Guideline indicates, and buffers (pH 1.2 and 6.8) - must be studied. To this end, a new stability-indicating micellar liquid chromatography (MLC) method has been optimised and validated. Measurements were based on the disappearance of reagents and the appearance of the only degradation product (AZT). This optimised and validated method used a C18 column and a mobile phase containing 0.05M sodium dodecyl sulphate-1% (v/v)1-butanol-0.01M NaH2PO4 (pH 3.0) at 30°C, and a flow rate of 1mLmin-1. Under these conditions, retention times were 1.4, 3.6, 6.3 and 9.5min for AZT-Ac, AZT, AZT-Val and AZT-Leu, respectively. Calibrations better than 0.9995, intra- and inter-day precisions below 1.08% and good recoveries (94.47-116.52%) and robustness (RSD less that 1.08%) were obtained and were adequate to analyse the four compounds. Finally, this MLC method was applied to achieve stability studies which resulted in the evidence that all the compounds followed a pseudo-first-order kinetics, and in the determination of their kinetic constants and half-life time. A reference method, applied in the same studies, validated the MLC method reported herein.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
GASTRIC AND INTESTINAL MATRICES
dc.subject
MICELLAR LIQUID CHROMATOGRAPHY
dc.subject
STABILITY-INDICATING HPLC METHODS
dc.subject
ZIDOVUDINE DERIVATIVES
dc.subject.classification
Otras Ciencias Químicas
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Stability-indicating micellar liquid chromatography method for three novel derivatives of zidovudine in aqueous and simulated gastric and intestinal fluids matrices
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-03-10T14:28:52Z
dc.journal.volume
1218
dc.journal.number
18
dc.journal.pagination
2540-2545
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Raviolo, Mónica Ana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Esteve Romero, Josep. Universitat Jaume I; España
dc.description.fil
Fil: Briñon, Margarita Cristina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
dc.journal.title
Journal of Chromatography - A
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0021967311002135
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.chroma.2011.02.018
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