Artículo
Lipid-based microtubes for topical delivery of Amphotericin B
Salerno, Claudia; Chiappetta, Diego Andrés
; Arechavala, Alicia; Gorlzaczany, Susana; Scioscia, Silvia L.; Bregni, Carlos
Fecha de publicación:
01/07/2013
Editorial:
Elsevier
Revista:
Colloids and Surfaces B: Biointerfaces
ISSN:
0927-7765
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The self-assembly process is a valuable tool for constructing nano and microstructures. Microtubes (MTs)self-assembled from amphiphiles are novel promising nanomaterials as they have easy self-assembly in aqueous solutions, reproducibility and biocompatibility. The incorporation of amphotericin B (AmB)into lipid microtubes formed from 12-hydroxystearic acid (12HSA) when mixed with ethanolamine in aqueous media was investigated. MTs of several concentrations of lipid material and AmB were prepared. The structure was characterized by phase-contrast microscopy, TEM and SEM. The type of interaction was analyzed by FTIR and DSC. Stability studies were carried out at room temperature and at 4 ◦C. Loading efficiency of the system was found to be much higher than the drug solubility in water. MTs with 1% of 12HSA and 1 mg/ml of AmB showed to be the most stable formulation. In vitro skin penetration assay showed a flux of 18.20 ± 3.35 g/cm2. Amb-loaded MTs in vitro antifungal activity was evaluated and formulation showed similar results to that of AmB deoxycholate showing that AmB retained its antifungal activity in the MTs formulation.
Palabras clave:
Lipid Microtubes
,
Self-Assembly
,
Amphotericin B
,
Topical Delivery
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Colecciones
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Salerno, Claudia; Chiappetta, Diego Andrés; Arechavala, Alicia; Gorlzaczany, Susana; Scioscia, Silvia L.; et al.; Lipid-based microtubes for topical delivery of Amphotericin B; Elsevier; Colloids and Surfaces B: Biointerfaces; 107; 1-7-2013; 160-166
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