Effect of inoculum age on kinetic of biomass formation and phenolic accumulations in Larrea divaricata Cav. cell suspension culture

Abstract

This work focuses on the analysis of the effect of inoculum age on the kinetic of biomass formation and phenolic accumulation in Larrea divaricata Cav. cell suspension culture. L. divaricata plant cell cultures (callus and cell suspension culture) successfully produce interesting phenolic compounds, being the main the lignan Nordihydroguaiaretic acid. The findings revealed that the inoculum age is an important factor on the kinetic biomass formation and phenolic compounds production in L. divaricata cell suspension culture. Four to seven month - inoculum age were suitable to establishment cell suspension culture, not only for biomass but also for the phenolic compounds production. On the contrary, when the age of the callus was over eighth month, no stimulation of phenolic compounds biosynthesis was observed.