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dc.contributor.author
Ordoñez, Omar Federico
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dc.contributor.author
Zannier, Federico
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dc.contributor.author
Albarracín, Virginia Helena
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dc.contributor.author
Farias, Maria Eugenia
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dc.date.available
2023-03-03T13:29:40Z
dc.date.issued
2015
dc.identifier.citation
The biofilms formation of exiguobacterium sp. S17 on synthetic supports and under the influence of arsenic; XI Congreso argentino de Microbiología General; Cordoba; Argentina; 2015; 1-2
dc.identifier.uri
http://hdl.handle.net/11336/189491
dc.description.abstract
The high-altitude Andean Lakes (HAAL) areecosystems located in the South American Andes. These ecosystems are unique dueto their geographical characteristics, their broad range of extremeenvironments, as well their abundant biodiversity. Thegenus Exiguobacteriumis one of the most widespread and representative genera on the HAAL, beingdetected by direct (pure culture isolation) and indirect (DGGE) techniques. Thisgenera have been isolated or molecularly detected from a wide range of habitatsincluding cold and hot environments with temperature between-12 and 55°C. This fact confers substantial interest to the genus as apotential model system to research attributes that may correlate withadaptation and evolution of organisms to diverse thermalregimes. Exiguobacterium sp. S17 is a high arsenic resistantpolyextremophilic bacteria isolated from the stromatolites of L. Socompa. This strain is able to grow readily in laboratory and represents an attractive model system for the study of environmentalstress. Previous studies showed that Exiguobacterium sp. S17 is able to resistto high arsenic concentration and to produce biofilm. The aim of this work was toassess biofilms formation byExiguobacterium sp. S17 in different synthetic supports and to investigatethe influence of arsenic (As[III] y As[V]) in their development.Determination andquantification of biofilms was measured using crystal violet 1% following themethodology proposed by Tomaras et al., (2003). Biofilms production wasevaluated at different incubation times (24, 48 and 72 h) in LB50media (without As) and in different synthetic supports: sterile glass tubes (15x 125mm) and polypropylene (12 x 75 mm) and polystyrene plates (20 cm3).The influence of As was investigated supplemented LB50 with arsenate(As[V]): 50mM, 100 mM, 150 mM, 200mM, 250mM and arsenite (As[III]): 2.5 mM,5mM, 7.5 mM, 10 mM, 12.5 mM at the same time.ANOVA analyzes revealed that the optimal production of biofilms isachieved after 24 hours of growth and the highest biofilm production wasobtained when using glass as support and adding arsenate (As [V]100 mM). No significant differences were observed when adding arsenite in comparison tocontrol medium (without arsenic).The findings obtained in this work made an important contribution to theknowledge of the biology and ecology of the microbial communities of the HAALin response to stress factors. Moreover, this method can be applied for thebenefit of human and environmental health by establishing an experimental basisfor a bioremediation method. Furthermore, we propose that HAAL is a source of novelbacterial species of biotechnological interest.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sociedad Argentina de Microbiologia General
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Arsenic resistance
dc.subject
Exiguobacterium
dc.subject
Biofilms
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The biofilms formation of exiguobacterium sp. S17 on synthetic supports and under the influence of arsenic
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-02-27T15:57:57Z
dc.journal.pagination
1-2
dc.journal.pais
Argentina
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dc.journal.ciudad
Cordoba
dc.description.fil
Fil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Zannier, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2015.pdf
dc.conicet.rol
Autor
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Autor
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Autor
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Autor
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dc.coverage
Nacional
dc.type.subtype
Congreso
dc.description.nombreEvento
XI Congreso argentino de Microbiología General
dc.date.evento
2015-08-05
dc.description.ciudadEvento
Cordoba
dc.description.paisEvento
Argentina
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dc.type.publicacion
Book
dc.description.institucionOrganizadora
Sociedad Argentina de Microbiología General
dc.source.libro
XI Congreso argentino de Microbiología General
dc.date.eventoHasta
2015-08-07
dc.type
Congreso
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