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Azzam, Aladdin  
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Solleh, Ramli  
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Roslinda Abd, Malek  
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Nor Zalina, Othman  
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Soad Abubakr, Abdelgalil  
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Dib, Julian Rafael  
dc.contributor.author
Hesham A., El Enshasy  
dc.date.available
2023-02-28T15:16:31Z  
dc.date.issued
2016  
dc.identifier.citation
Statistical optimization of medium components for wickerhamomyces anomalus killer toxin production; 6th International Conference on Biotechnology for The Wellness Industry; Melaka; Malasia; 2016; 1-2  
dc.identifier.uri
http://hdl.handle.net/11336/189103  
dc.description.abstract
Yeast killer toxin (KT) was associated with the killer system group where yeast is able to synthesize and export proteins or glycoproteins with toxic effects to control citrus postharvest diseases. In this study strains belonging to the species Kazachstania exigua, Saccharomyces cerevisiae and Wickerhamomyces anomalus isolated from citrus plants of Tucuman city at Argentina were screening in vitro as a potential biocontrol agents (BCAs) against of Aspergillus niger. W. anomalus was chosen to further develop and established to ensure good growth with high activity cells for killer toxin used for industrial application. Medium formulation is an essential stage in the design of pilot scale development in order to optimize (KT) production with the medium must satisfy the elemental requirement for (KT) biosynthesis and cell maintenance. Therefore, the aim of this study is to enhance production of (KT) by applying two-stage statistical approach, Plackett-Burman design and response surface methodology (RSM) using central composite design (CCD) of the medium composition through the shake flask levels. In the first stage of optimization, among the studied variables, peptone, glycerol, and pH were selected based on their high positive significant effect on the productivity of (KT). The second stage of experimental design (RSM) using (CCD) was utilized to create a polynomial quadratic model correlating the relationship between the three variables and the productivity of (KT). The optimal combination of the major medium constituents for (KT) production evaluated using the non-linear optimization algorithm of EXCEL-Solver and JMP program, was as follows: 6% peptone, 30% glycerol and pH 4. The predicted optimum production of (KT) against A. niger was 1904 arbitrary units (AU) per ml via Plackett-Burman design. By using the optimal fermentation medium of (RSM) design as follows 6.69%, 15.1% at pH 4.3, the (KT) production was improved approximate two times higher over the previous production in the un-optimized medium.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
UniversitiTeknologi Malaysia  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
KILLER TOXIN  
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Plackett-Burman  
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Wickerhamomyces anomalus  
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Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.  
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Biotecnología Industrial  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Statistical optimization of medium components for wickerhamomyces anomalus killer toxin production  
dc.type
info:eu-repo/semantics/publishedVersion  
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info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2023-02-15T17:27:52Z  
dc.journal.pagination
1-2  
dc.journal.pais
Malasia  
dc.journal.ciudad
Johor Bahru  
dc.description.fil
Fil: Azzam, Aladdin. UniversitiTeknologi Malaysia; Malasia  
dc.description.fil
Fil: Solleh, Ramli. UniversitiTeknologi Malaysia; Malasia  
dc.description.fil
Fil: Roslinda Abd, Malek. UniversitiTeknologi Malaysia; Malasia  
dc.description.fil
Fil: Nor Zalina, Othman. UniversitiTeknologi Malaysia; Malasia  
dc.description.fil
Fil: Soad Abubakr, Abdelgalil. Genetic Engineering and Biotechnology Research Institute; Egipto  
dc.description.fil
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Hesham A., El Enshasy. UniversitiTeknologi Malaysia; Malasia  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ibd.utm.my/icbwi16/  
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dc.coverage
Internacional  
dc.type.subtype
Conferencia  
dc.description.nombreEvento
6th International Conference on Biotechnology for The Wellness Industry  
dc.date.evento
2016-08-16  
dc.description.ciudadEvento
Melaka  
dc.description.paisEvento
Malasia  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
UniversitiTeknologi Malaysia  
dc.source.libro
6th International Conference on Biotechnology for The Wellness Industry  
dc.date.eventoHasta
2016-08-17  
dc.type
Conferencia