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dc.contributor.author
Azzam, Aladdin
dc.contributor.author
Solleh, Ramli
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Roslinda Abd, Malek
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Nor Zalina, Othman
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Soad Abubakr, Abdelgalil
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Dib, Julian Rafael
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Hesham A., El Enshasy
dc.date.available
2023-02-28T15:16:31Z
dc.date.issued
2016
dc.identifier.citation
Statistical optimization of medium components for wickerhamomyces anomalus killer toxin production; 6th International Conference on Biotechnology for The Wellness Industry; Melaka; Malasia; 2016; 1-2
dc.identifier.uri
http://hdl.handle.net/11336/189103
dc.description.abstract
Yeast killer toxin (KT) was associated with the killer system group where yeast is able to synthesize and export proteins or glycoproteins with toxic effects to control citrus postharvest diseases. In this study strains belonging to the species Kazachstania exigua, Saccharomyces cerevisiae and Wickerhamomyces anomalus isolated from citrus plants of Tucuman city at Argentina were screening in vitro as a potential biocontrol agents (BCAs) against of Aspergillus niger. W. anomalus was chosen to further develop and established to ensure good growth with high activity cells for killer toxin used for industrial application. Medium formulation is an essential stage in the design of pilot scale development in order to optimize (KT) production with the medium must satisfy the elemental requirement for (KT) biosynthesis and cell maintenance. Therefore, the aim of this study is to enhance production of (KT) by applying two-stage statistical approach, Plackett-Burman design and response surface methodology (RSM) using central composite design (CCD) of the medium composition through the shake flask levels. In the first stage of optimization, among the studied variables, peptone, glycerol, and pH were selected based on their high positive significant effect on the productivity of (KT). The second stage of experimental design (RSM) using (CCD) was utilized to create a polynomial quadratic model correlating the relationship between the three variables and the productivity of (KT). The optimal combination of the major medium constituents for (KT) production evaluated using the non-linear optimization algorithm of EXCEL-Solver and JMP program, was as follows: 6% peptone, 30% glycerol and pH 4. The predicted optimum production of (KT) against A. niger was 1904 arbitrary units (AU) per ml via Plackett-Burman design. By using the optimal fermentation medium of (RSM) design as follows 6.69%, 15.1% at pH 4.3, the (KT) production was improved approximate two times higher over the previous production in the un-optimized medium.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
UniversitiTeknologi Malaysia
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
KILLER TOXIN
dc.subject
Plackett-Burman
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Wickerhamomyces anomalus
dc.subject.classification
Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Statistical optimization of medium components for wickerhamomyces anomalus killer toxin production
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-02-15T17:27:52Z
dc.journal.pagination
1-2
dc.journal.pais
Malasia
dc.journal.ciudad
Johor Bahru
dc.description.fil
Fil: Azzam, Aladdin. UniversitiTeknologi Malaysia; Malasia
dc.description.fil
Fil: Solleh, Ramli. UniversitiTeknologi Malaysia; Malasia
dc.description.fil
Fil: Roslinda Abd, Malek. UniversitiTeknologi Malaysia; Malasia
dc.description.fil
Fil: Nor Zalina, Othman. UniversitiTeknologi Malaysia; Malasia
dc.description.fil
Fil: Soad Abubakr, Abdelgalil. Genetic Engineering and Biotechnology Research Institute; Egipto
dc.description.fil
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Hesham A., El Enshasy. UniversitiTeknologi Malaysia; Malasia
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ibd.utm.my/icbwi16/
dc.conicet.rol
Autor
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Autor
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dc.conicet.rol
Autor
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Autor
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Autor
dc.coverage
Internacional
dc.type.subtype
Conferencia
dc.description.nombreEvento
6th International Conference on Biotechnology for The Wellness Industry
dc.date.evento
2016-08-16
dc.description.ciudadEvento
Melaka
dc.description.paisEvento
Malasia
dc.type.publicacion
Book
dc.description.institucionOrganizadora
UniversitiTeknologi Malaysia
dc.source.libro
6th International Conference on Biotechnology for The Wellness Industry
dc.date.eventoHasta
2016-08-17
dc.type
Conferencia
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