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dc.contributor.author
Valinotto, Laura Elena
dc.contributor.author
Diez, Roberto Alejandro
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Barrero, Paola Roxana
dc.contributor.author
Farías, Julio A.
dc.contributor.author
López, Eduardo L.
dc.contributor.author
Mistchenko, Alicia Susana
dc.date.available
2023-02-27T14:23:04Z
dc.date.issued
2010-08
dc.identifier.citation
Valinotto, Laura Elena; Diez, Roberto Alejandro; Barrero, Paola Roxana; Farías, Julio A.; López, Eduardo L.; et al.; Emergence of intratreatment resistance to oseltamivir in pandemic influenza A H1N1 2009 virus; SAGE Publications; Antiviral Therapy.; 15; 6; 8-2010; 923-927
dc.identifier.issn
1359-6535
dc.identifier.uri
http://hdl.handle.net/11336/188964
dc.description.abstract
Background: Pandemic influenza A H1N1 2009 virus presents a new challenge to health authorities and communities worldwide. In Argentina, the outbreak was at its peak by the end of June 2009, during the southern winter. A systematic analysis of samples from patients with pandemic H1N1 2009 studied in our laboratory (Virology Laboratory, Hospital de Niños R Gutiérrez, Buenos Aires, Argentina) detected two patients presenting intratreatment emergence of the H275Y neuraminidase mutation, which confers resistance to oseltamivir. Methods: Complementary DNAs, including the 275 codon, were obtained by reverse transcriptase PCR using viral RNAs extracted from nasopharyngeal or tracheal aspirates. Conventional sequencing and pyrosequencing were performed on each sample. In order to measure the virus susceptibility to oseltamivir, 50% inhibitory concentration determinations were performed by chemiluminescence. Results: Sequential samples of two paediatric patients under oseltamivir treatment were analysed. Pretreatment samples were composed of 100% oseltamivir-sensitive variants. In case 1, the oseltamivir-resistant variant was found 8 days after the beginning of treatment. In case 2, the viral population became resistant on the second day of treatment, with 83% of the viral population bearing the mutation and this reached 100% on the seventh day. Conclusions: We describe the intratreatment emergence of oseltamivir resistance in two paediatric patients. Pyrosequencing allowed us to detect variant mixtures, showing the transition of the viral population from sensitive to resistant.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
SAGE Publications
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
INFLUENZA A H1N1 2009
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NEURAMINIDASE
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RESISTANCE
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OSELTAMIVIR
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Virología
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Emergence of intratreatment resistance to oseltamivir in pandemic influenza A H1N1 2009 virus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-02-20T14:11:14Z
dc.journal.volume
15
dc.journal.number
6
dc.journal.pagination
923-927
dc.journal.pais
Estados Unidos
dc.journal.ciudad
California
dc.description.fil
Fil: Valinotto, Laura Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
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Fil: Diez, Roberto Alejandro. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Biosidus S. A.; Argentina
dc.description.fil
Fil: Barrero, Paola Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil
Fil: Farías, Julio A.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil
Fil: López, Eduardo L.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil
Fil: Mistchenko, Alicia Susana. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
dc.journal.title
Antiviral Therapy.
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.3851/IMP1635
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3851/IMP1635
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