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dc.contributor.author
Powlesland, Alex S.
dc.contributor.author
Barrio, Maria Marcela
dc.contributor.author
Mordoh, Jose
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Hitchen, Paul G
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Dell, Anne
dc.contributor.author
Drickamer, Kurt
dc.contributor.author
Taylor, Maureen E
dc.date.available
2023-02-24T12:52:37Z
dc.date.issued
2011-03
dc.identifier.citation
Powlesland, Alex S.; Barrio, Maria Marcela; Mordoh, Jose; Hitchen, Paul G; Dell, Anne; et al.; Glycoproteomic characterization of carriers of the CD15/Lewisx epitope on Hodgkin's Reed-Sternberg cells; BioMed Central; Bmc Biochemistry; 12; 1; 3-2011; 13-13
dc.identifier.issn
1471-2091
dc.identifier.uri
http://hdl.handle.net/11336/188776
dc.description.abstract
Background: The Lewisx trisaccharide, also referred to as the CD15 antigen, is a diagnostic marker used to distinguish Hodgkin's lymphoma from other lymphocytic cancers. However, the role of such fucosylated structures remains poorly understood, in part because carriers of Lewisx structures on Hodgkin's Reed-Sternberg cells have not been identified. Methods. GalMBP, an engineered carbohydrate-recognition protein that binds selectively to oligosaccharides with paired terminal galactose and fucose residues, has been used in conjunction with proteomic and glycomic analysis to identify glycoprotein carriers of Lewisx and related glycan structures in multiple Hodgkin's Reed-Sternberg cell lines. Results: Multiple glycoproteins that bind to GalMBP and carry CD15/Lewisx have been identified in a panel of six Reed-Sternberg cell lines. The most commonly identified Lewis x-bearing glycoproteins are CD98hc, which was found in all six cell lines tested, and intercellular adhesion molecule-1 and DEC-205, which were detected in five and four of the lines, respectively. Thus, several of the most prominent cell adhesion molecules on the lymphomas carry this characteristic glycan epitope. In addition, the Hodgkin's Reed-Sternberg cell lines can be grouped into subsets based on the presence or absence of less common Lewis x-bearing glycoproteins. Conclusions: CD98 and intercellular adhesion molecule-1 are major carriers of CD15/Lewisx on Reed-Sternberg cells. Binding of DC-SIGN and other glycan-specific receptors to the Lewis x epitopes on CD98 and intercellular adhesion molecule-1 may facilitate interaction of the lymphoma cells with lymphocytes and myeloid cells in lymph nodes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
GalMBP
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CD15/Lewis X
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Hodgkin´s Reed Sternberg cells
dc.subject.classification
Bioquímica y Biología Molecular
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Glycoproteomic characterization of carriers of the CD15/Lewisx epitope on Hodgkin's Reed-Sternberg cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-02-21T22:15:15Z
dc.journal.volume
12
dc.journal.number
1
dc.journal.pagination
13-13
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Powlesland, Alex S.. Imperial College London; Reino Unido
dc.description.fil
Fil: Barrio, Maria Marcela. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: Hitchen, Paul G. Imperial College London; Reino Unido
dc.description.fil
Fil: Dell, Anne. Imperial College London; Reino Unido
dc.description.fil
Fil: Drickamer, Kurt. Imperial College London; Reino Unido
dc.description.fil
Fil: Taylor, Maureen E. Imperial College London; Reino Unido
dc.journal.title
Bmc Biochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bmcbiochem.biomedcentral.com/articles/10.1186/1471-2091-12-13
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/1471-2091-12-13
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