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dc.contributor.author
Quiroga, Ezequiel Dario
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dc.contributor.author
Alvarez, María Gabriela
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dc.contributor.author
Durantini, Edgardo Néstor
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dc.date.available
2023-02-23T01:46:21Z
dc.date.issued
2010-07
dc.identifier.citation
Quiroga, Ezequiel Dario; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Susceptibility of Candida albicans to photodynamic action of 5,10,15,20-tetra(4-N-methylpyridyl)porphyrin in different media; Wiley Blackwell Publishing, Inc; Fems Immunology And Medical Microbiology; 60; 2; 7-2010; 123-131
dc.identifier.issn
0928-8244
dc.identifier.uri
http://hdl.handle.net/11336/188647
dc.description.abstract
The photodynamic activity of 5,10,15,20-tetra(4-N-methylpyridyl)porphyrin (TMPyP) was evaluated in vitro on Candida albicans cells under different experimental conditions. This tetracationic porphyrin binds rapidly to C. albicans cells, reaching a value of ~1.7 nmol 10-6 cells when the cellular suspensions (106 CFU mL-1) were incubated with 5 μM sensitizer. The amount of cell-bound sensitizer is not appreciably changed when cultures are incubating for longer times (>15 min) but it diminishes with the number of washing steps. Photosensitized inactivation of C. albicans cellular suspensions increases with both sensitizer concentration and irradiation time, causing a ~5 log decrease of cell survival when the cultures are treated with 5 μM TMPyP and irradiated for 30 min. However, the photocytotoxicity decreases after one washing step, with the decrease in cell-bound sensitizer. The growth of C. albicans cells was arrested when the cultures were exposed to 5 μM TMPyP and visible light. On agar surfaces, the phototoxic effect of this sensitizer, which caused an inactivation of C. albicans cells, remained high. No growth was observed in areas containing TMPyP and irradiated. Moreover, in small C. albicans colonies, C. albicans cells were completely inactivated. These studies indicate that TMPyP is an effective sensitizer for photodynamic inactivation of yeasts in both liquid suspensions or localized on a surface.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CATIONIC PORPHYRIN
dc.subject
PHOTODYNAMIC INACTIVATION
dc.subject
PHOTOSENSITIZERS
dc.subject
CANDIDA ALBICANS
dc.subject.classification
Otras Ciencias de la Salud
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dc.subject.classification
Ciencias de la Salud
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Susceptibility of Candida albicans to photodynamic action of 5,10,15,20-tetra(4-N-methylpyridyl)porphyrin in different media
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-02-02T23:11:03Z
dc.journal.volume
60
dc.journal.number
2
dc.journal.pagination
123-131
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Quiroga, Ezequiel Dario. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Durantini, Edgardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; Argentina
dc.journal.title
Fems Immunology And Medical Microbiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1111/j.1574-695x.2010.00725.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/60/2/123/635580?login=false
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