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dc.contributor.author
Hollmann, Axel  
dc.contributor.author
Delfederico, Lucrecia  
dc.contributor.author
de Antoni, Graciela Liliana  
dc.contributor.author
Semorile, Liliana Carmen  
dc.contributor.author
Disalvo, Edgardo Anibal  
dc.date.available
2023-02-17T12:32:50Z  
dc.date.issued
2010-08  
dc.identifier.citation
Hollmann, Axel; Delfederico, Lucrecia; de Antoni, Graciela Liliana; Semorile, Liliana Carmen; Disalvo, Edgardo Anibal; Interaction of bacterial surface layer proteins with lipid membranes: Synergysm between surface charge density and chain packing; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 79; 1; 8-2010; 191-197  
dc.identifier.issn
0927-7765  
dc.identifier.uri
http://hdl.handle.net/11336/188342  
dc.description.abstract
S-layer proteins from Lactobacillus kefir and Lactobacillus brevis are able to adsorb on the surface of positively charged liposomes composed by Soybean lecithin, cholesterol and stearylamine. The different K values for S-layer proteins isolated from L. kefir and L. brevis (4.22×10-3 and 2.45×102μM-1 respectively) indicates that the affinity of the glycosylated protein isolated from L. kefir is higher than the non-glycosylated one.The attachment of S-layer proteins counteracts the electrostatic charge repulsion between stearylamine molecules in the membrane surface, producing an increase in the rigidity in the acyl chains as measured by DPH anisotropy. Laurdan generalized polarization (GP) shows that glycosylated causes a GP increase, attributed to a lowering in water penetration into the head groups of membrane phospholipids, with charge density reduction, while the non-glycosylated does not affect it.The octadecyl-rhodamine results indicate that S-layer coated liposomes do not show spontaneous dequenching in comparison with control liposomes without S-layer proteins, suggesting that S-layer protein avoid spontaneous liposomal fusion.It is concluded that the increase in stability of liposomes coated with S-layers proteins is due to the higher rigidity induced by the S-layer attachment by electrostatic forces.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
DPH  
dc.subject
LAURDAN  
dc.subject
LIPOSOMES  
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OCTADECYL-RHODAMINE  
dc.subject
S-LAYER PROTEINS FROM LACTOBACILLI  
dc.subject.classification
Biofísica  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Interaction of bacterial surface layer proteins with lipid membranes: Synergysm between surface charge density and chain packing  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-02-16T13:29:52Z  
dc.journal.volume
79  
dc.journal.number
1  
dc.journal.pagination
191-197  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Hollmann, Axel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Delfederico, Lucrecia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: de Antoni, Graciela Liliana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina  
dc.description.fil
Fil: Semorile, Liliana Carmen. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina  
dc.description.fil
Fil: Disalvo, Edgardo Anibal. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.journal.title
Colloids and Surfaces B: Biointerfaces  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0927776510001943  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org//10.1016/j.colsurfb.2010.03.046