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Alfaro, Juan Manuel  
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Reyes, Sarita Isabel  
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Martearena, Maria Rita  
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Blanco, Silvia Cristina  
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Baigori, Mario Domingo  
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Pera, Licia Maria  
dc.date.available
2023-02-16T11:45:57Z  
dc.date.issued
2017  
dc.identifier.citation
Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa; XII Congreso Argentino de Microbiología General; San Miguel de Tucumán; Argentina; 2017; 1-4  
dc.identifier.uri
http://hdl.handle.net/11336/188205  
dc.description.abstract
The cell wall is a complex and dynamic structure. It is also a site of diverse enzyme activities. The balance between its synthesis and lysis is critical, since wall synthesis in the absence of lysis can cause excessive wall thickening and possible growth arrest; while, lysis in the absence of synthesis would produce bursting of the cell. Therefore, within permissible limits, the net balance between wall synthesis and wall lysis will exert a marked influence on the cell growth and morphology. In this work, the b-D-glucosidase activity was firstly selected as a marker of the wall lytic potential of both Rhodotorula glutinis and Rhodotorula mucilaginosa using p-nitrophenyl-β-D-glucopyranoside as substrate. Secondly, a doses-response study involving several metal ions was conducted; as a result, the FeCl3 and MnCl2 significant increased the biomass-bound β-D-glucosidase activity from R. glutinis and R. mucilaginosa, respectively. Thirdly, to test the influence of each metal ion, 6 mM of either FeCl3 (for R. mucilaginosa) or MnCl2 (for R. glutinis) was supplemented to a medium with lower nitrogen content (MLN). The MLN without any addition was taken as the control. Cultures were incubated at 30°C and 180 rpm during six days. Thus, the major and minor diameter of R. mucilaginosa increased from 3.40 ± 0.23 μm to 5.28 ± 0.40 μm and from 2.60 ± 0.23 μm to 5.20 ± 0.55 μm, respectively, in the presence of 6 mM FeCl3. And, the major and minor diameter of R. glutinis increased from 4,90 ± 0.47 μm to 5.48 ± 0.42 μm and from 2.23 ± 0.53 μm to 4.95 ± 0.49 μm, respectively, in the presence of 6 mM MnCl2. So, from these results it might be concluded that the supplementation of either FeCl3 or MnCl2 to the culture medium induced morphological changes in both oleaginous yeasts. In addition, the biomass and lipid concentration obtained after fermentations were also discussed.  
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application/pdf  
dc.language.iso
eng  
dc.publisher
Asociación Civil de Microbiología General  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
RHODOTORULA GLUTINIS  
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RHODOTORULA MUCILAGINOSA  
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METAL IONS  
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GLUCOSIDASE  
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CELL MORPHOLOGY  
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Bioprocesamiento Tecnológico, Biocatálisis, Fermentación  
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Biotecnología Industrial  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Influence of metal ions on β-D-glucosidase and cell morphology of Rhodotorula glutinis and Rhodotorula mucilaginosa  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2023-02-10T17:55:04Z  
dc.journal.pagination
1-4  
dc.journal.pais
Argentina  
dc.journal.ciudad
San Miguel de Tucumán  
dc.description.fil
Fil: Alfaro, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina  
dc.description.fil
Fil: Reyes, Sarita Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina  
dc.description.fil
Fil: Martearena, Maria Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina  
dc.description.fil
Fil: Blanco, Silvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina  
dc.description.fil
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2017.pdf  
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Nacional  
dc.type.subtype
Congreso  
dc.description.nombreEvento
XII Congreso Argentino de Microbiología General  
dc.date.evento
2017-08-02  
dc.description.ciudadEvento
San Miguel de Tucumán  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Asociación Civil de Microbiología General  
dc.source.libro
XII Congreso Argentino de Micorbiología General  
dc.date.eventoHasta
2017-08-04  
dc.type
Congreso