Mostrar el registro sencillo del ítem
dc.contributor.author
Canal Martinez, Veronica de Los Angeles
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Baigori, Mario Domingo
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Pera, Licia Maria
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2023-02-14T14:29:56Z
dc.date.issued
2017
dc.identifier.citation
Production of a lipase activity by solid state fermentation using Aspergillus niger MYA 135: Impact of different humectant mixtures; XII Congreso Argentino de Microbiología General; San Miguel de Tucumán; Argentina; 2017; 1-4
dc.identifier.uri
http://hdl.handle.net/11336/187926
dc.description.abstract
The solid state fermentation is an economical alternative for production of industrial enzymes such as lipases mainly because this technology proposes the reuse of agro-industrial waste as well as adding value to those residues. Lipases (EC 3.1.1.3) are triacylglycerol hydrolases that catalyze the hydrolysisof triglycerides to free fatty acids and glycerol. In non-conventional systems these enzymes can also catalyze esterification, transesterification and interesterification reactions. The aim of this work was to evaluate the production of a lipase activity from Aspergillus niger MYA 135 by solid state fermentation using different humectant mixtures. As solid substrate, washed sugarcane bagasse was used. Humidity was adjusted to 90 % with the following mixtures containing (in g/l): 1) M1: Vinasse 5.0, molasses 5.0, milk serum 10.0, peptone 5.0, cerelose 5.0, waste frying oil 10.0, FeCl3 1.0, CaCl2 0.5. 2) M2: Sucrose 10.0, KH2PO4 1.0, NH4NO3 2.0, MgSO4 0.2, CuSO4 0.06, FeCl3 1, olive oil 20. 3) M3: Sucrose 10.0, KH2PO4 1.0, NH4NO3 2.0, MgSO4 0.2, CuSO4 0.06, yeast extract 1.0, peptone 5.0, olive oil 20. Humectant mixtures M1 and M2 were previously used as culture media for A. niger MYA 135 lipase production by submerged fermentation. Humectant mixture M3 was formulated from literature data. Reactors were inoculated with 105 conidia per gram of solid substrate and incubated at 30°C during 48 h. Then, the fermented substrate was dried at 45 °C and used as enzyme source. The hydrolytic activity was measured with p-nitrophenyl palmitate (C16) as substrate. The molar extinction coefficientof p-nitrophenol (p-NP) under the given assay conditions was 0.0073 μM-1 cm-1. One unit of enzyme activity (U) was defined as the amount of biocatalyst that released 1 μmol of p-NP per minute. Specific activity was expressed as Unit per gram of dry fermented substrate (U/gdfs). All experiments were performedin triplicate and analyzed with the Minitab software for Windows. Under our assays conditions, the specific lipase activity obtained from sugarcane bagasse supplemented with M1, M2 and M3 were 626.7, 978.1 and 252.8 U/gdfs, respectively. In addition, the performance of the biocatalysts producedby submerged fermentation using the same liquid mixtures was also discussed.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Asociación Argentina de Microbiología
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ASPERGILLUS NIGER
dc.subject
SOLID STATE FERMENTATION
dc.subject
LIPASE ACTIVITY
dc.subject
HUMECTANT MIXTURES
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Biotecnología Industrial
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Production of a lipase activity by solid state fermentation using Aspergillus niger MYA 135: Impact of different humectant mixtures
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-02-10T17:55:00Z
dc.journal.pagination
1-4
dc.journal.pais
Argentina
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
San Miguel de Tucumán
dc.description.fil
Fil: Canal Martinez, Veronica de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2017.pdf
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.coverage
Nacional
dc.type.subtype
Congreso
dc.description.nombreEvento
XII Congreso Argentino de Microbiología General
dc.date.evento
2017-08-02
dc.description.ciudadEvento
San Miguel de Tucumán
dc.description.paisEvento
Argentina
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.type.publicacion
Book
dc.description.institucionOrganizadora
Asociación Argentina de Microbiología
dc.source.libro
XII Congreso Argentino de Micorbiología General
dc.date.eventoHasta
2017-08-04
dc.type
Congreso
Archivos asociados