Artículo
Picobirnavirus causes persistent infection in pigs
Martinez, Laura Cecilia; Masachessi, Gisela
; Carruyo, Gabriela; Ferreyra, Leonardo Jesús; Barril, Patricia Angelica
; Isa, Maria Beatriz; Giordano, Miguel Oscar; Ludert, Juan E.; Nates, Silvia Viviana
Fecha de publicación:
10/2010
Editorial:
Elsevier Science
Revista:
Infection, Genetics and Evolution
ISSN:
1567-1348
e-ISSN:
1567-7257
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A study aimed to further understand the biology of porcine picobirnaviruses (PBV) was conducted between November 2003 and January 2008, on a farm located in the outskirts of Córdoba City, Argentina. PBV prevalence was examined by polyacrylamide gel electrophoresis and silver staining (PAGE S/S) on a total of 265 samples collected from pigs divided into four groups, according to age and physiological status. PBV detection rate was highest in the group of sows sampled within the lactogenic period (38.02%; p<0.05), followed by pregnant sows (15.09%), piglets aged 2-5 months of age (18.42%) and adult (≥50 weeks) male pigs (0%). In addition, 103 samples collected in 3 follow-up studies were analyzed by PAGE S/S and reverse transcription followed by PCR (RT-PCR). Two of these studies followed female pigs from weaning up to slaughter and a third one from weaning up to 4 pregnancy periods. The results provide evidence that PBV establishes a persistent infection in the host with periods of silence intermingled with periods of low and high viral excretion. High PBV excretion levels were detected by PAGE S/S and were conditioned by age (primary infection) and host physiological status. Low PBV excretion levels were detected by RT-PCR throughout the entire study period. Sequence analysis of selected amplicons indicated that the virus excreted through the follow-up study was the same. These results suggest that porcine PBV is maintained in nature by transmission from infected asymptomatic individuals to susceptible ones.
Palabras clave:
EXCRETION
,
PERSISTENT INFECTION
,
PICOBIRNAVIRUS
,
PORCINE
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Identificadores
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Martinez, Laura Cecilia; Masachessi, Gisela; Carruyo, Gabriela; Ferreyra, Leonardo Jesús; Barril, Patricia Angelica; et al.; Picobirnavirus causes persistent infection in pigs; Elsevier Science; Infection, Genetics and Evolution; 10; 7; 10-2010; 984-988
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