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dc.contributor.author
Schneck, Emanuel
dc.contributor.author
Schubert, Thomas
dc.contributor.author
Konovalov, Oleg V.
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Quinn, Bonnie E.
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Gutsmann, Thomas
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Brandenburg, Klaus
dc.contributor.author
Oliveira, Rafael Gustavo
dc.contributor.author
Pink, David A.
dc.contributor.author
Tanaka, Motomu
dc.date.available
2023-02-06T10:32:19Z
dc.date.issued
2010-05
dc.identifier.citation
Schneck, Emanuel; Schubert, Thomas; Konovalov, Oleg V.; Quinn, Bonnie E.; Gutsmann, Thomas; et al.; Quantitative determination of ion distributions in bacterial lipopolysaccharide membranes by grazing-incidence X-ray fluorescence; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 107; 20; 5-2010; 9147-9151
dc.identifier.issn
0027-8424
dc.identifier.uri
http://hdl.handle.net/11336/186931
dc.description.abstract
A model of the outer membrane of Gram-negative bacteria was created by the deposition of a monolayer of purified rough mutant lipopolysaccharides at an air/water interface. The density profiles of monovalent (K+) and divalent (Ca2+) cations normal to the lipopolysaccharides (LPS) monolayers were investigated using grazingincidence X-ray fluorescence. In the absence of Ca2+, a K+ concentration peak was found in the negatively charged LPS headgroup region. With the addition of CaCl2, Ca2+ ions almost completely displaced K+ ions from the headgroup region. By integrating the experimentally reconstructed excess ion density profiles, we obtained an accurate measurement of the effective charge density of LPS monolayers. The experimental findings were compared to the results of Monte Carlo simulations based on a coarse-grained minimal model of LPS molecules and showed excellent agreement.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
National Academy of Sciences
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BIOLOGICAL INTERFACE
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ELECTROSTATICS
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MONOLAYER
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MONTE CARLO SIMULATION
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Quantitative determination of ion distributions in bacterial lipopolysaccharide membranes by grazing-incidence X-ray fluorescence
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-01-31T12:38:32Z
dc.identifier.eissn
1091-6490
dc.journal.volume
107
dc.journal.number
20
dc.journal.pagination
9147-9151
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Schneck, Emanuel. Ruprecht Karls Universitat Heidelberg; Alemania
dc.description.fil
Fil: Schubert, Thomas. Ruprecht Karls Universitat Heidelberg; Alemania
dc.description.fil
Fil: Konovalov, Oleg V.. European Synchrotron Radiation Facility; Francia
dc.description.fil
Fil: Quinn, Bonnie E.. Saint Francis Xavier University; Canadá
dc.description.fil
Fil: Gutsmann, Thomas. Research Center Borstel; Alemania
dc.description.fil
Fil: Brandenburg, Klaus. Research Center Borstel; Alemania
dc.description.fil
Fil: Oliveira, Rafael Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.description.fil
Fil: Pink, David A.. Saint Francis Xavier University; Canadá
dc.description.fil
Fil: Tanaka, Motomu. Ruprecht Karls Universitat Heidelberg; Alemania
dc.journal.title
Proceedings of the National Academy of Sciences of The United States of America
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.pnas.org/doi/suppl/10.1073/pnas.0913737107
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1073/pnas.0913737107
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