Artículo
Cross regulation between Candida albicans catalytic and regulatory subunits of protein kinase A
Giacometti, Romina
; Kronberg, Maria Florencia
; Biondi, Ricardo Miguel
; Hernandez, Alejandra Ines
; Passeron, Susana
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Fecha de publicación:
01/2012
Editorial:
Elsevier
Revista:
Fungal Genetics And Biology
ISSN:
1087-1845
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In the pathogen Candida albicans protein kinase A (PKA) catalytic subunit is encoded by two genes TPK1 and TPK2 and the regulatory subunit by one gene, BCY1. PKA mediates several cellular processes such as cell cycle regulation and the yeast to hyphae transition, a key factor for C. albicans virulence. The catalytic isoforms Tpk1p and Tpk2p share redundant functions in vegetative growth and hyphal development, though they differentially regulate glycogen metabolism, the stress response pathway and pseudohyphal formation. In Saccharomyces cerevisiae it was earlier reported that BCY1 overexpression not only increased the amount of TPK3 mRNA but also its catalytic activity. In C. albicans a significant decrease in Bcy1p expression levels was already observed in tpk2Δ null strains. In this work we showed that the upregulation in Bcy1p expression was observed in a set of strains having a TPK1 or TPK2 allele reintegrated in its own locus, as well as in strains expressing the TPKs under the control of the constitutive ACT1 promoter. To confirm the cross regulation event between Bcy1p and Tpkp expression we generated a mutant strain with the lowest PKA activity carrying one TPK1 and a unique BCY1 allele with the aim to obtain two derived strains in which BCY1 or TPK1 were placed under their own promoters inserted in the RPS10 neutral locus. We found that placing one copy of BCY1 upregulated the levels of Tpk1p and its catalytic activity; while TPK1 insertion led to an increase in BCY1 mRNA, Bcy1p and in a high cAMP binding activity. Our results suggest that C. albicans cells were able to compensate for the increased levels of either Tpk1p or Tpk2p subunits with a corresponding elevation of Bcy1 protein levels and vice versa, implying a tightly regulated mechanism to balance holoenzyme formation.
Palabras clave:
Tpk
,
Candida
,
Bcy1
,
Cross-Regulation
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Articulos(INBA)
Articulos de INST.DE INVEST. EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Articulos de INST.DE INVEST. EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Citación
Giacometti, Romina; Kronberg, Maria Florencia; Biondi, Ricardo Miguel; Hernandez, Alejandra Ines; Passeron, Susana; Cross regulation between Candida albicans catalytic and regulatory subunits of protein kinase A; Elsevier; Fungal Genetics And Biology; 49; 1; 1-2012; 74-85
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