Alternative method with amperometric detection for ranitidine determination

Abstract

An alternative, simple, and reliable FIA amperometric method for ranitidine quantification was developed. A working glassy carbon electrode and a Ag|AgCl|NaCl (3 M) reference electrode were used. The determination is based on the electrochemical oxidation of ranitidine at 1.2 V in phosphate buffer solution (pH 7.2). The determination of ranitidine in the range of 1-10 6 to 2-105 M was possible with a sampling frequency of 120 h-1. The method was validated and satisfactorily applied to the determination of ranitidine in pharmaceutical formulations with a low limit of detection (6-107 M) and a quantitative recovery with no interferences from excipients. The results compare favorably with those obtained by the liquid chromatographic method of the U.S. Pharmacopoeia. © 2010 American Chemical Society.