Artículo
Dectin-1 on macrophages modulates the immune response to Fasciola hepatica products through the ERK signaling pathway
Guasconi, Lorena
; Burstein, Verónica Liliana
; Beccacece, Ignacio
; Mena, Cristian Javier
; Chiapello, Laura Silvina
; Masih, Diana Teresa
Fecha de publicación:
12/2018
Editorial:
Elsevier Gmbh
Revista:
Immunobiology
ISSN:
0171-2985
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Fasciolosis is a zoonotic disease of increasing importance due to its worldwide distribution and elevated economic losses. Previously, we demonstrated that Fasciola hepatica excretory–secretory products (FhESP) induce immunomodulatory effects on peritoneal macrophages in a Dectin-1 dependent manner. In this study, we observed that peritoneal macrophages from naive BALB/c mice stimulated in vitro with FhESP presented increased expression levels of phosphorylated extracellular-signal-regulated kinase (ERK), and this effect was dependent on Syk, protein kinase C (PKC) and Dectin-1. In this sense, we observed increased levels of arginase activity, IL-10 and TGF-β in macrophages stimulated with FhESP, which were dependent on PKC and ERK. Furthermore, we observed that the increased arginase activity, as well as in TGF-β and IL-10 levels, was partially dependent on IL-10 receptor signaling in macrophages that were pre-incubated with anti-IL10R before being stimulated with FhESP. Taken together, these results suggest the participation of Dectin-1 and Syk in FhESP interaction with peritoneal macrophages and the possible role of ERK and IL-10 in downstream signaling pathways involved in the immunomodulatory effects induced by Fasciola hepatica products.
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Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Guasconi, Lorena; Burstein, Verónica Liliana; Beccacece, Ignacio; Mena, Cristian Javier; Chiapello, Laura Silvina; et al.; Dectin-1 on macrophages modulates the immune response to Fasciola hepatica products through the ERK signaling pathway; Elsevier Gmbh; Immunobiology; 223; 12; 12-2018; 834-838
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