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dc.contributor.author
Sanchez, María Belén  
dc.contributor.author
Moreno Sosa, María Tamara  
dc.contributor.author
Neira, Flavia Judith  
dc.contributor.author
Pietrobon, Elisa Olivia  
dc.contributor.author
Soaje, Marta  
dc.contributor.author
Valdez, Susana Ruth  
dc.contributor.author
Jahn, Graciela Alma  
dc.contributor.author
Mackern Oberti, Juan Pablo  
dc.date.available
2023-01-30T14:05:35Z  
dc.date.issued
2019  
dc.identifier.citation
Thyroxine excess increases fetal growth, placental and milk immune cell infiltration in late pregnancy and early lactation; XXXVII Reunión Anual Científica de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 1-4  
dc.identifier.uri
http://hdl.handle.net/11336/186092  
dc.description.abstract
Thyroid dysfunctions cause reproductive disorders that lead to preterm birth and profound endocrine alterations during lactation with deficient milk production and quality. Currently, whether thyroid hormones (THs) modulate immune cells in maternal milk and placenta is unknown. Therefore, the aim of our work was to assess the influence of hyperthyroidism (hyper) on placental immune cells as well as its impact in pregnancy and lactation. To this end, 10-12 weeks old Wistar rats were injected with a daily dose of T4 (0.1 or 0.25 mg/kg s.c) to induce hyper, or vehicle in control animals. Rats were mated 8 days after starting T4 treatment and euthanized on day 19 (G19), 20 (G20) of gestation and 2 of lactation (L2). Placenta samples and milk were minced to reach single cell suspension and dyed. Then, resident placental and milk immune cells (CD45+, CD3+, CD11b/c+) were analyzed by flow cytometry and mRNA content of hormone receptors by qPCR. Also, placental (G19-20), fetus (G19-20) and offspring weights were measured. The fetuses of hyper 0.25 mg/kg mothers weighed more in G19 and G20 (p<0,001). The placentas of the hyper 0.25 mg/kg mothers were heavier than controls only in G19 (p<0,001). Furthermore, we showed a decrease in the expression of progesterone, estrogen and β2 thyroid receptors in hyper 0.1 mg/kg (p<0.05). On G19, the percentage of placental leukocytes was significantly higher in both hyper groups (p<0.05). On G20 we showed an increase in leukocyte infiltrate compared with G19 in the control (p<0.001) but not in the hyper group. We observed that hyper mothers had a higher mortality rate than the control group (14,14% and 3,18% respectively). Furthermore, the hyper group offspring presented lower weight on days 1 and 2 (p<0,001). Rat milk had an increase in the percentage of CD45+ cells in the Hyper (p<0,05). In addition, the absolute quantity of CD3+ cells/ µl increased in respect to the control group while the number of CD11 b/c+ diminished (p<0,05). These results suggest that T4 administration accelerates fetal development, changes placental sensitivity to ovarian steroids and impairs early lactation. Additionally, placental and milk leukocytes would be modulated by T4. To our knowledge, this is the first report that shows the modulation of resident immune cells by thyroid hormones.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Socidad de Biología de Cuyo  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
thyroxine  
dc.subject
T cells  
dc.subject
placenta  
dc.subject
lactation  
dc.subject.classification
Inmunología  
dc.subject.classification
Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Thyroxine excess increases fetal growth, placental and milk immune cell infiltration in late pregnancy and early lactation  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2022-12-12T23:47:54Z  
dc.journal.pagination
1-4  
dc.journal.pais
Argentina  
dc.journal.ciudad
San Luis  
dc.description.fil
Fil: Sanchez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina  
dc.description.fil
Fil: Moreno Sosa, María Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina  
dc.description.fil
Fil: Neira, Flavia Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina  
dc.description.fil
Fil: Pietrobon, Elisa Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina  
dc.description.fil
Fil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina  
dc.description.fil
Fil: Valdez, Susana Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina  
dc.description.fil
Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina  
dc.description.fil
Fil: Mackern Oberti, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/12/Libro-BIOCELL-2019.pdf  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.coverage
Nacional  
dc.type.subtype
Reunión  
dc.description.nombreEvento
XXXVII Reunión Anual Científica de la Sociedad de Biología de Cuyo  
dc.date.evento
2019-12-05  
dc.description.ciudadEvento
San Luis  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Sociedad de Biología de Cuyo  
dc.source.libro
Libro de Resúmenes: XXXVII Reunión Científica Anual Sociedad de Biología de Cuyo  
dc.date.eventoHasta
2019-12-06  
dc.type
Reunión