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dc.contributor.author
Loste, Nicolás
dc.contributor.author
Nestares, Graciela María
dc.contributor.author
Felitti, Silvina Andrea
dc.contributor.author
Ochogavía, Ana Claudia
dc.date.available
2023-01-27T18:19:43Z
dc.date.issued
2022-01
dc.identifier.citation
Loste, Nicolás; Nestares, Graciela María; Felitti, Silvina Andrea; Ochogavía, Ana Claudia; Gene expression profiling by cDNA-AFLP reveals potential candidate genes for imazapyr-induced male sterility in sunflower; John Wiley & Sons; Crop Science; 62; 1; 1-2022; 301-314
dc.identifier.issn
0011-183X
dc.identifier.uri
http://hdl.handle.net/11336/185995
dc.description.abstract
Chemically induced male sterility (CIMS) is a valuable tool for hybrid breeding in crops. In this context, the acetohydroxyacid synthase (AHAS)-inhibiting imazapyr has been proposed to induce male sterility in Imisun sunflower (Helianthus annuus L.) when applied during early reproductive development in a two-fold higher than recommended field dose. This study aimed to perform a comparative transcriptomic analysis to find out the molecular bases of the gametocidal effect of imazapyr on two Imisun genotypes differing in their herbicide resistance level: completely resistant and intermediate resistant. The CIMS of treated plants was confirmed at physiological and anatomical levels. A total of 948 differentially expressed transcript-derived fragments (TDFs) were identified by complementary DNA amplified fragment-length polymorphism (cDNA-AFLP) analysis at the three most informative developmental stages of anther development. Several genes related to xenobiotic metabolism and stress, including cytochrome P450 monooxygenases and ATP-binding cassette transporters, were identified in the completely resistant genotype. Interestingly, a group of genes, including protein kinases, sulfonylurea receptor 1, aspartic signal peptide-peptidase, and specific membrane transporters, were recognized from the differential TDFs simultaneously detected in both genotypes. Some of these transcripts were validated by quantitative real-time polymerase chain reaction (qPCR) in both genotypes. Therefore, these transcripts could be exclusively related to the CIMS in sunflower. Based on the results, we suggest a probable model of action for the gametocidal effect of imazapyr. Our research provides new insights into the molecular mechanisms of male sterility induction in sunflower.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AHAS
dc.subject
CHEMICAL INDUCED MALE-STERILITY
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TRANSCRIPT DERIVED FRAGMENTS
dc.subject
ACETOHYDROXYACID SYNTHASE
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SUNFLOWER
dc.subject.classification
Otras Biotecnología Agropecuaria
dc.subject.classification
Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Gene expression profiling by cDNA-AFLP reveals potential candidate genes for imazapyr-induced male sterility in sunflower
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-07T18:17:24Z
dc.identifier.eissn
1435-0653
dc.journal.volume
62
dc.journal.number
1
dc.journal.pagination
301-314
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva Jersey
dc.description.fil
Fil: Loste, Nicolás. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
dc.description.fil
Fil: Nestares, Graciela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
dc.description.fil
Fil: Felitti, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
dc.description.fil
Fil: Ochogavía, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
dc.journal.title
Crop Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://acsess.onlinelibrary.wiley.com/doi/10.1002/csc2.20640
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1002/csc2.20640
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