Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Evento

Brain vascular alterations during the postnatal period in a rat model of intrauterine growth restriction

Asensio, Joana AntonelaIcon ; García, Rodrigo DamiánIcon ; Seltzer, Alicia MabelIcon
Tipo del evento: Reunión
Nombre del evento: Sociedad Argentina de Fisiología + Asociación Latino Americana de Ciencias Fisiológicas 2021 Joint Meeting: Discovering the physiological gears from evolution to Translation
Fecha del evento: 20/10/2021
Institución Organizadora: Sociedad Argentina de Fisiología; Asociación Latino Americana de Ciencias Fisiológicas;
Título de la revista: Physiological Mini Reviews
Editorial: Latin American Association of Physiological Sciences
ISSN: 1669-5410
Idioma: Inglés
Clasificación temática:
Otras Medicina Básica

Resumen

Introduction: Spontaneously hypertensive rats (SHR) endure pregnancy with placental insufficiency, resulting in chronic fetal hypoxia and postnatal neurodevelopmental delay. SHR pups have been proposed as a model for intrauterine growth restriction (IUGR). Impaired cerebrovascular development is strongly related to alterations in the neurovascular unit (NVU). Caveolin-1 (CAV-1) is associated with microvessels, astrocytes and pericytes, the main components of the NVU and it is associated with the tissue response to hypoxic injuries. Objectives: To evaluate brain vascular development during the postnatal period in SHR pups.Methods: SHR and Wistar Kyoto (WKY) pups at P7 and P14 were used to obtain cerebral cortex and cerebellum histological slices. Masson?s trichrome (MT) was performed to detect arterioles; and CAV-1 / IB4 double fluorescent-staining for NVU. We used Image J and AngioTool software to determine the following variables: total number of junctions (TNJ), total number of endpoints(TNEp), vessels area (VA), average vessels length (AVL), lacunarity index (LAC) and ratios IB4/CAV-1. Unpaired Student t-test used for Statistical analysis, the results were reported as Mean ± SEM. Results: Cerebral cortex and cerebellum from SHR P14 presented a significant increase in the number of arterioles. WKY cortex from P7 to P14 manifested a significant increase in TNJ, TNE, andVA; and a decrease in LAC. Only TNE and VPA increased significantly in SHR from P7 to P14. In SHR cortex P14, TVL, and TNEp there were significant differences between ratio CAV-1/IB4, where IB4 had higher expression. In LAC, the situation was reversed. Cerebellum P7 and P14, there were no significant differences in CAV-1 and IB4.Conclusions: The microvascular development of the SHR brain was characterized by an increased number of arterioles, high lacunarity index and poor capillary network. These data together with the altered CAV-1/IB4 ratio suggest a probable delay in NVU consolidation.
Palabras clave: SPONTANEOUSLY HYPERTENSIVE RATS , VASCULAR DEVELOPMENT , CAVEOLIN - 1 , ISOLECTIN B4
Ver el registro completo
 
Archivos asociados
Tamaño: 690.5Kb
Formato: PDF
.
Solicitar
Licencia
info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/185947
URL: https://pmr.safisiol.org.ar/volume/volume-14/
Colecciones
Eventos(IMBECU)
Eventos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Citación
Brain vascular alterations during the postnatal period in a rat model of intrauterine growth restriction; Sociedad Argentina de Fisiología + Asociación Latino Americana de Ciencias Fisiológicas 2021 Joint Meeting: Discovering the physiological gears from evolution to Translation; La Plata; Argentina; 2021; 49-49
Compartir

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • X Conicet Digital
  • Instagram
  • YouTube
  • Sound Cloud
  • LinkedIn

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES