Artículo
Time-Resolved NMR Analysis of Proteolytic α-Synuclein Processing in vitro and in cellulo
Limatola, Antonio; Eichmann, Cédric; Jacob, Reeba Susan; Ben-Nissan, Gili; Sharon, Michal; Binolfi, Andrés
; Selenko, Philipp
Fecha de publicación:
11/2018
Editorial:
Wiley VCH Verlag
Revista:
Proteomics (weinheim. Print)
ISSN:
1615-9853
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Targeted proteolysis of the disordered Parkinson's disease protein alpha-synuclein (αSyn) constitutes an important event under physiological and pathological cell conditions. In this work, site-specific αSyn cleavage by different endopeptidases in vitro and by endogenous proteases in extracts of challenged and unchallenged cells was studied by time-resolved NMR spectroscopy. Specifically, proteolytic processing was monitored under neutral and low pH conditions and in response to Rotenone-induced oxidative stress. Further, time-dependent degradation of electroporation-delivered αSyn in intact SH-SY5Y and A2780 cells was analyzed. Results presented here delineate a general framework for NMR-based proteolysis studies in vitro and in cellulo, and confirm earlier reports pertaining to the exceptional proteolytic stability of αSyn under physiological cell conditions. However, experimental findings also reveal altered protease susceptibilities in selected mammalian cell lines and upon induced cell stress.
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Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Limatola, Antonio; Eichmann, Cédric; Jacob, Reeba Susan; Ben-Nissan, Gili; Sharon, Michal; et al.; Time-Resolved NMR Analysis of Proteolytic α-Synuclein Processing in vitro and in cellulo; Wiley VCH Verlag; Proteomics (weinheim. Print); 18; 21-22; 11-2018; 1-13
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