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dc.contributor.author
Hüdig, Meike
dc.contributor.author
Tronconi, Marcos Ariel
dc.contributor.author
Zubimendi, Juan Pablo
dc.contributor.author
Sage, Tammy L.
dc.contributor.author
Poschmann, Gereon
dc.contributor.author
Bickel, David
dc.contributor.author
Gohlke, Holger
dc.contributor.author
Maurino, Verónica
dc.date.available
2023-01-26T17:51:11Z
dc.date.issued
2022-01
dc.identifier.citation
Hüdig, Meike; Tronconi, Marcos Ariel; Zubimendi, Juan Pablo; Sage, Tammy L.; Poschmann, Gereon; et al.; Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cell mitochondria and evolved via association of differentially adapted subunits; American Society of Plant Biologist; Plant Cell; 34; 1; 1-2022; 597-615
dc.identifier.issn
1040-4651
dc.identifier.uri
http://hdl.handle.net/11336/185818
dc.description.abstract
In plant mitochondria, nicotinamide adenine dinucleotide-malic enzyme (NAD-ME) has a housekeeping function in malate respiration. In different plant lineages, NAD-ME was independently co-opted in C4 photosynthesis. In the C4 Cleome species, Gynandropsis gynandra and Cleome angustifolia, all NAD-ME genes (NAD-MEa, NAD-MEb1, and NAD-MEb2) were affected by C4 evolution and are expressed at higher levels than their orthologs in the C3 species Tarenaya hassleriana. In T. hassleriana, the NAD-ME housekeeping function is performed by two heteromers, NAD-MEa/b1 and NAD-MEa/b2, with similar biochemical properties. In both C4 species, this role is restricted to NAD-MEa/b2. In the C4 species, NAD-MEa/b1 is exclusively present in the leaves, where it accounts for most of the enzymatic activity. Gynandropsis gynandra NAD-MEa/b1 (GgNAD-MEa/b1) exhibits high catalytic efficiency and is differentially activated by the C4 intermediate aspartate, confirming its role as the C4-decarboxylase. During C4 evolution, NAD-MEb1 lost its catalytic activity; its contribution to the enzymatic activity results from a stabilizing effect on the associated a-subunit and the acquisition of regulatory properties. We conclude that in bundle sheath cell mitochondria of C4 species, the functions of NAD-ME as C4 photosynthetic decarboxylase and as a housekeeping enzyme coexist and are performed by isoforms that combine the same a-subunit with differentially adapted b-subunits.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society of Plant Biologist
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
C4 plants
dc.subject
NAD-ME
dc.subject
Evolution
dc.subject
Regulatory site
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cell mitochondria and evolved via association of differentially adapted subunits
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-20T11:05:27Z
dc.identifier.eissn
1532-298X
dc.journal.volume
34
dc.journal.number
1
dc.journal.pagination
597-615
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Rockville
dc.description.fil
Fil: Hüdig, Meike. Universitat Bonn; Alemania
dc.description.fil
Fil: Tronconi, Marcos Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
dc.description.fil
Fil: Zubimendi, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
dc.description.fil
Fil: Sage, Tammy L.. University of Toronto; Canadá
dc.description.fil
Fil: Poschmann, Gereon. Heinrich Heine University Düsseldorf; Alemania
dc.description.fil
Fil: Bickel, David. Heinrich Heine University Düsseldorf; Alemania
dc.description.fil
Fil: Gohlke, Holger. Heinrich Heine University Düsseldorf; Alemania
dc.description.fil
Fil: Maurino, Verónica. Universitat Bonn; Alemania
dc.journal.title
Plant Cell
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1093/plcell/koab265
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/article/34/1/597/6420711?login=false
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