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dc.contributor.author
Raimunda, Daniel Cesar
dc.contributor.author
Long, Jarukit E.
dc.contributor.author
Padilla Benavides, Teresita
dc.contributor.author
Sassetti, Christopher M.
dc.contributor.author
Argüello, José M.
dc.date.available
2023-01-26T13:15:25Z
dc.date.issued
2014-01
dc.identifier.citation
Raimunda, Daniel Cesar; Long, Jarukit E.; Padilla Benavides, Teresita; Sassetti, Christopher M.; Argüello, José M.; Differential roles for the Co2+/Ni2+ transporting ATPases, CtpD and CtpJ, in Mycobacterium tuberculosis virulence; Wiley Blackwell Publishing, Inc; Molecular Microbiology; 91; 1; 1-2014; 185-197
dc.identifier.issn
0950-382X
dc.identifier.uri
http://hdl.handle.net/11336/185746
dc.description.abstract
The genome of Mycobacterium tuberculosis encodes two paralogous P1B4-ATPases, CtpD (Rv1469) and CtpJ (Rv3743). Both proteins showed ATPase activation by Co2+ and Ni2+, and both appear to be required for metal efflux from the cell. However, using a combination of biochemical and genetic studies we found that these proteins play non-redundant roles in virulence and metal efflux. CtpJ expression is induced by Co2+ and this protein possesses a relatively high turnover rate. A ctpJ deletion mutant accumulated Co2+, indicating that this ATPase controls cytoplasmic metal levels. In contrast, CtpD expression is induced by redox stressors and this protein displays a relatively low turnover rate. A ctpD mutant failed to accumulate metal, suggesting an alternative cellular function. ctpD is cotranscribed with two thioredoxin genes trxA (Rv1470), trxB (Rv1471), and an enoyl-coA hydratase (Rv1472), indicating a possible role for CtpD in the metallation of these redox-active proteins. Supporting this, in vitro metal binding assays showed that TrxA binds Co2+ and Ni2+. Mutation of ctpD, but not ctpJ, reduced bacterial fitness in the mouse lung, suggesting that redox maintenance, but not Co2+ accumulation, is important for growth in vivo.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CtpD
dc.subject
CtpJ
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Co2+-ATPase
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Redox stress
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Differential roles for the Co2+/Ni2+ transporting ATPases, CtpD and CtpJ, in Mycobacterium tuberculosis virulence
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-01-19T11:23:40Z
dc.identifier.eissn
1365-2958
dc.journal.volume
91
dc.journal.number
1
dc.journal.pagination
185-197
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Raimunda, Daniel Cesar. Worcester Polytechnic Institute; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.description.fil
Fil: Long, Jarukit E.. Massachusetts Institute of Technology; Estados Unidos
dc.description.fil
Fil: Padilla Benavides, Teresita. Worcester Polytechnic Institute; Estados Unidos
dc.description.fil
Fil: Sassetti, Christopher M.. Worcester Polytechnic Institute; Estados Unidos
dc.description.fil
Fil: Argüello, José M.. Worcester Polytechnic Institute; Estados Unidos
dc.journal.title
Molecular Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/mmi.12454
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/mmi.12454
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