Artículo
Conservation of zebrafish microrna-145 and its role during neural crest cell development
Steeman, Tomás José
; Rubiolo, Juan A.; Sánchez, Laura E.; Calcaterra, Nora Beatriz
; Weiner, Andrea Maria Julia
Fecha de publicación:
07/2021
Editorial:
MDPI
Revista:
Genes
ISSN:
2073-4425
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The neural crest is a multipotent cell population that develops from the dorsal neural fold of vertebrate embryos in order to migrate extensively and differentiate into a variety of tissues. A number of gene regulatory networks coordinating neural crest cell specification and differentiation have been extensively studied to date. Although several publications suggest a common role for microRNA-145 (miR-145) in molecular reprogramming for cell cycle regulation and/or cellular differentiation, little is known about its role during in vivo cranial neural crest development. By modifying miR-145 levels in zebrafish embryos, abnormal craniofacial development and aberrant pigmentation phenotypes were detected. By whole-mount in situ hybridization, changes in expression patterns of col2a1a and Sry-related HMG box (Sox) transcription factors sox9a and sox9b were observed in overexpressed miR-145 embryos. In agreement, zebrafish sox9b expression was downregulated by miR-145 overexpression. In silico and in vivo analysis of the sox9b 3′UTR revealed a conserved potential miR-145 binding site likely involved in its post-transcriptional regulation. Based on these findings, we speculate that miR-145 participates in the gene regulatory network governing zebrafish chondrocyte differentiation by controlling sox9b expression.
Palabras clave:
EMBRYONIC DEVELOPMENT
,
GENE REGULATORY NETWORK
,
MICRORNA
,
NEURAL CREST
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Identificadores
Colecciones
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Steeman, Tomás José; Rubiolo, Juan A.; Sánchez, Laura E.; Calcaterra, Nora Beatriz; Weiner, Andrea Maria Julia; Conservation of zebrafish microrna-145 and its role during neural crest cell development; MDPI; Genes; 12; 7; 7-2021; 1-21
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