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dc.contributor.author
Capella, Matias
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dc.contributor.author
Caballero, Lucía Martín
dc.contributor.author
Pfander, Boris
dc.contributor.author
Braun, Sigurd
dc.contributor.author
Jentsch, Stefan
dc.date.available
2023-01-23T15:17:21Z
dc.date.issued
2020-12
dc.identifier.citation
Capella, Matias; Caballero, Lucía Martín; Pfander, Boris; Braun, Sigurd; Jentsch, Stefan; ESCRT recruitment by the S. Cerevisiae inner nuclear membrane protein Heh1 is regulated by hub1-mediated alternative splicing; Company of Biologists; Journal of Cell Science; 133; 24; 12-2020; 1-12
dc.identifier.issn
0021-9533
dc.identifier.uri
http://hdl.handle.net/11336/185282
dc.description.abstract
Misassembled nuclear pore complexes (NPCs) are removed by sealing off the surrounding nuclear envelope (NE), which is conducted by the endosomal sorting complexes required for transport (ESCRT) machinery. Recruitment of ESCRT proteins to the NE is mediated by the interaction between the ESCRT member Chm7 and the inner nuclear membrane protein Heh1, which belongs to the conserved LEM family. Increased ESCRT recruitment results in excessive membrane scission at damage sites but its regulation remains poorly understood. Here, we show that Hub1-mediated alternative splicing of HEH1 pre-mRNA, resulting in production of its shorter form Heh1-S, is critical for the integrity of the NE in Saccharomyces cerevisiae. ESCRT-III mutants lacking Hub1 or Heh1-S display severe growth defects and accumulate improperly assembled NPCs. This depends on the interaction of Chm7 with the conserved MSC domain, which is only present in the longer variant Heh1-L. Heh1 variants assemble into heterodimers, and we demonstrate that a unique splice segment in Heh1-S suppresses growth defects associated with the uncontrolled interaction between Heh1-L and Chm7. Together, our findings reveal that Hub1-mediated splicing generates Heh1-S to regulate ESCRT recruitment to the NE.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Company of Biologists
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ESCRT
dc.subject
LEM DOMAIN
dc.subject
NUCLEAR ENVELOPE SURVEILLANCE
dc.subject
NUCLEAR PORE COMPLEX
dc.subject
SPLICING
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
ESCRT recruitment by the S. Cerevisiae inner nuclear membrane protein Heh1 is regulated by hub1-mediated alternative splicing
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-01-23T00:35:22Z
dc.journal.volume
133
dc.journal.number
24
dc.journal.pagination
1-12
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Capella, Matias. Max Planck Institute Of Biochemistry.; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Caballero, Lucía Martín. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Pfander, Boris. Max Planck Institute Of Biochemistry.; Alemania
dc.description.fil
Fil: Braun, Sigurd. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Jentsch, Stefan. Max Planck Institute Of Biochemistry.; Alemania
dc.journal.title
Journal of Cell Science
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jcs.biologists.org/lookup/doi/10.1242/jcs.250688
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1242/jcs.250688
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