Artículo
On the roles of wheat endosperm ADP-glucose pyrophosphorylase subunits
Ferrero, Danisa María Luján
; Asención Diez, Matías Damián
; Kuhn, Misty L.; Falaschetti, Christine A.; Piattoni, Claudia Vanesa
; Iglesias, Alberto Alvaro
; Ballicora, Miguel A.
Fecha de publicación:
10/2018
Editorial:
Frontiers Media
Revista:
Frontiers in Plant Science
e-ISSN:
1664-462X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The ADP-glucose pyrophosphorylase from wheat endosperm controls starch synthesis in seeds and has unique regulatory properties compared to others from this family. It comprises two types of subunits, but despite its importance little is known about their roles. Here, we synthesized de novo the wheat endosperm ADP-glucose pyrophosphorylase small (S) and large (L) subunit genes, heterologously expressed them in Escherichia coli, and kinetically characterized the recombinant proteins. To understand their distinct roles, we co-expressed them with well characterized subunits from the potato tuber enzyme to obtain hybrids with one S subunit from one source and an L subunit from the other. After kinetic analyses of these hybrids, we concluded that the unusual insensitivity to activation of the wheat endosperm enzyme is caused by a pre-activation of the L subunit. In addition, the heat stability and sensitivity to phosphate are given by the S subunit.
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Articulos(IAL)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Citación
Ferrero, Danisa María Luján; Asención Diez, Matías Damián; Kuhn, Misty L.; Falaschetti, Christine A.; Piattoni, Claudia Vanesa; et al.; On the roles of wheat endosperm ADP-glucose pyrophosphorylase subunits; Frontiers Media; Frontiers in Plant Science; 871; 10-2018; 1-7
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