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dc.contributor.author
Lei, Bingkun  
dc.contributor.author
Capella, Matias  
dc.contributor.author
Montgomery, Sean A.  
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Borg, Michael  
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Osakabe, Akihisa  
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Goiser, Malgorzata  
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Muhammad, Abubakar  
dc.contributor.author
Braun, Sigurd  
dc.contributor.author
Berger, Frédéric  
dc.date.available
2023-01-19T14:09:33Z  
dc.date.issued
2021-01  
dc.identifier.citation
Lei, Bingkun; Capella, Matias; Montgomery, Sean A.; Borg, Michael; Osakabe, Akihisa; et al.; A Synthetic Approach to Reconstruct the Evolutionary and Functional Innovations of the Plant Histone Variant H2A.W; Cell Press; Current Biology; 31; 1; 1-2021; 182-191.e5  
dc.identifier.issn
0960-9822  
dc.identifier.uri
http://hdl.handle.net/11336/185011  
dc.description.abstract
Diversification of histone variants is marked by the acquisition of distinct motifs and functional properties through convergent evolution.1–4 H2A variants are distinguished by specific C-terminal motifs and tend to be segregated within defined domains of the genome.5,6 Whether evolution of these motifs pre-dated the evolution of segregation mechanisms or vice versa has remained unclear. A suitable model to address this question is the variant H2A.W, which evolved in plants through acquisition of a KSPK motif7 and is tightly associated with heterochromatin.4 We used fission yeast, where chromatin is naturally devoid of H2A.W, to study the impact of engineered chimeras combining yeast H2A with the KSPK motif. Biochemical assays showed that the KSPK motif conferred nucleosomes with specific properties. Despite uniform incorporation of the engineered H2A chimeras in the yeast genome, the KSPK motif specifically affected heterochromatin composition and function. We conclude that the KSPK motif promotes chromatin properties in yeast that are comparable to the properties and function of H2A.W in plant heterochromatin. We propose that the selection of functional motifs confer histone variants with properties that impact primarily a specific chromatin state. The association between a new histone variant and a preferred chromatin state can thus provide a setting for the evolution of mechanisms that segregate the new variant to this state, thereby enhancing the impact of the selected properties of the variant on genome activity.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Cell Press  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
CHROMATIN  
dc.subject
EVOLUTION  
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H2A.M  
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H2A.W  
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HETEROCHROMATIN  
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HISTONE  
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HISTONE VARIANT  
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STREPTOPHYTES  
dc.subject.classification
Bioquímica y Biología Molecular  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
A Synthetic Approach to Reconstruct the Evolutionary and Functional Innovations of the Plant Histone Variant H2A.W  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2022-09-21T16:17:58Z  
dc.journal.volume
31  
dc.journal.number
1  
dc.journal.pagination
182-191.e5  
dc.journal.pais
Estados Unidos  
dc.description.fil
Fil: Lei, Bingkun. Gregor Mendel Institute; Austria  
dc.description.fil
Fil: Capella, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina  
dc.description.fil
Fil: Montgomery, Sean A.. Gregor Mendel Institute; Austria  
dc.description.fil
Fil: Borg, Michael. Gregor Mendel Institute; Austria  
dc.description.fil
Fil: Osakabe, Akihisa. Gregor Mendel Institute; Austria  
dc.description.fil
Fil: Goiser, Malgorzata. Gregor Mendel Institute; Austria  
dc.description.fil
Fil: Muhammad, Abubakar. Universitat Technical Zu Munich; Alemania  
dc.description.fil
Fil: Braun, Sigurd. Universitat Technical Zu Munich; Alemania  
dc.description.fil
Fil: Berger, Frédéric. Gregor Mendel Institute; Austria  
dc.journal.title
Current Biology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0960982220314913  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.cub.2020.09.080