Evento
New bacteriocin of E. Faecalis CECT7121: synergy studies against multidrug-resistant enterococci isolated from bovine mastitis
Delpech, Gastón
; Ceci, Monica Andrea; Bistoletti, Mariana
; Sanchez Bruni, Sergio Fabian
; Sparo, Mónica Delfina
Tipo del evento:
Reunión
Nombre del evento:
LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Sociedad Argentina de Farmacología Experimental, VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Fecha del evento:
15/11/2016
Institución Organizadora:
Sociedad Argentina de Investigación Clínica;
Sociedad Argentina de Inmunología;
Sociedad Argentina de Farmacología Experimental;
Sociedad Argentina de Nanomedicina;
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio;
Título de la revista:
Medicina (Buenos Aires)
Editorial:
Fundación Revista Medicina
ISSN:
0025-7680
Idioma:
Inglés
Clasificación temática:
Resumen
Bovine mastitis impacts negatively on animal production and on the quality of milk. AP-CECT7121, a new bacteriocin produced by Enterococcus faecalis CECT7121, shows a variety of interesting biological properties with potential use in clinical Veterinary practice, without undesirable side effects. It is a peptide with a wide spectrum of bactericidal activity against Gram positive bacteria and bacteriostatic activity against some Gram negative bacteria. The aim of this study was to evaluate the synergism of AP-CECT7121 associated with gentamicin against multidrug-resistant enterococci isolated from bovine mastitis. N: 4 multidrug-resistant E. faecium strains isolated from different mastitic dairy cows were tested. Animals from dairy farms, located in the Province of Buenos Aires-Argentina, during the period 2014-2015 were included. AP-CECT7121 potency was assessed by time-kill curves alone or with sub-inhibitory concentrations of gentamicin. High level gentamicin resistance was not observed in any enterococcal isolate. Viable counts, after 0, 2, 4, 8 and 24 h of incubation, were carried out. The AP-CECT7121exhibited bactericidal activity alone against all tested enterococci. The association with gentamicin slightly enhanced bactericidal activity against all isolates of E .faecium. Bactericidal activity of AP-CECT7121 alone and associated with subinhibitory concentrations of gentamicin against enterococci proved that this peptide constitute an attractive candidate for its use as a natural therapeutic tool for control and prevention of bovine mastitis produced by multi-resistant bacteria such as vancomycin-resistant E. faecium.
Palabras clave:
BACTERIA
,
RESISTANCE
,
PEPTIDE
Archivos asociados
Licencia
Identificadores
Colecciones
Eventos(CIVETAN)
Eventos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Eventos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Citación
New bacteriocin of E. Faecalis CECT7121: synergy studies against multidrug-resistant enterococci isolated from bovine mastitis; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Sociedad Argentina de Farmacología Experimental, VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2016; 276-276
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