Mostrar el registro sencillo del ítem
dc.contributor.author
Campelo, Adrián Esteban
dc.contributor.author
Massheimer, Virginia Laura
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2023-01-11T18:27:14Z
dc.date.issued
2021
dc.identifier.citation
Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1
dc.identifier.uri
http://hdl.handle.net/11336/184400
dc.description.abstract
The obesity pandemic is a major worldwide health concern that predisposes to a higher risk of metabolic and cardiovascular diseases (CVD). In menopausal women the decline of ovarian steroidogenesis is associated to a high prevalence of CVD and obesity. Steroid hormones have a pivotal role in the regulation of angiogenesis. Vascularization of the adipose tissue modulates adipogenesis, lipids storage through a complex interplay not fully understood¬¬.According to intracrinology, DHEA can be converted into active sex steroids in peripheral tissues, avoiding their systemic exposure. DHEA supplementation is proposed as a low risk therapy for the prevention of postmenopausal diseases.The aim of this work was to study: a) the effect of DHEA administration on the metabolic profile and on oxidative stress markers related to obesity using using a murine model of obesity and hipoestrogenism; b) the role of DHEA on angiogenesis.Ovariectomized Wistar rats feed with standard diet (ND) (4%fat) or high fat (HF) diet (27%fat) received daily injections of vehicle (C) or DHEA (1mg/kg.day) for 8 weeks. Angiogenic effect of DHEA was evaluated in vitro using primary cultures of endothelial cells (EC) and ex vivo, using the rat aortic ring assay. Caloric intake was 22% higher in HF vs ND groups, with an increase of body weight and adiposity index. No significative differences were detected in glucose, cholesterol, HDL-cholesterol and triglycerides levels (table). In contrast, DHEA induced reduction in Cholesterol/HDL index in ND group, and of serum ROS (H2-DCFDA) both in NF and HF groups.Nitric oxide production ex vivo by rat aortic rings was enhanced by DHEA in both ND and HF (130% and 138% vs C, p<0.01), effect dependent on DHEA convertion to more active steroids since it was abolished in presence of a 3β-HSD inhibitor.The angiogenic process requires ECs proliferation, migration and organization. In ECs in vitro treatment with DHEA increased cell proliferation (130% above C p<0.01) and enhanced cell migration (15% above C p<0.05). Indeed, DHEA stimulated capillary tube formation when ECs were cultured in a fibrin matrix. Consistingly Ex vivo assays showed that DHEA (20 and 200nM) stimulated capillary tubes formation around aortic ring (8.5 vs 28.1, 29.2μm, C vs 20nM-200nM-DHEA p<0.05).These results suggest that DHEA supplementation to hipoestrogenic rats would improve metabolic and inflammatory markers associated with obesity, and also promotes neovascularization.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
International Society of Endocrinology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANDROGENS
dc.subject
OBESITY
dc.subject
OXIDATIVE STRESS
dc.subject
ANGIOGENESIS
dc.subject.classification
Endocrinología y Metabolismo
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Medicina Clínica
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Dehydroepiandrosterone supplementation improves cellular and biochemical markers of obesity
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2022-11-09T15:29:47Z
dc.journal.pagination
1-1
dc.journal.pais
Suiza
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Ginebra
dc.description.fil
Fil: Campelo, Adrián Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.description.fil
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.isendo.org/event/ice-virtual-congress-2021/
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.coverage
Internacional
dc.type.subtype
Congreso
dc.description.nombreEvento
19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”
dc.date.evento
2021-02-24
dc.description.ciudadEvento
Buenos Aires
dc.description.paisEvento
Argentina
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.type.publicacion
Book
dc.description.institucionOrganizadora
International Society of Endocrinology
dc.source.libro
The ICE 2021 Abstracts E-Book
dc.date.eventoHasta
2021-02-28
dc.type
Congreso
Archivos asociados