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Artículo

Effects of in vitro interactions of oviduct epithelial cells with frozen–thawed stallion spermatozoa on their motility, viability and capacitation status

Gimeno, Brenda Florencia; Bariani, Maria VictoriaIcon ; Laiz Quiroga, Lucía BelénIcon ; Martínez León, Eduardo AntonioIcon ; Von Meyeren, MicaelaIcon ; Rey, OsvaldoIcon ; Mutto, Adrián AngelIcon ; Osycka Salut, Claudia ElenaIcon
Fecha de publicación: 01/2021
Editorial: Multidisciplinary Digital Publishing Institute
Revista: Animals
ISSN: 2076-2615
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Reproductiva

Resumen

Cryopreservation by negatively affecting sperm quality decreases the efficiency of assisted reproduction techniques (ARTs). Thus, we first evaluated sperm motility at different conditions for the manipulation of equine cryopreserved spermatozoa. Higher motility was observed when spermatozoa were incubated for 30 min at 30 × 106 /mL compared to lower concentrations (p < 0.05) and when a short centrifugation at 200× g was performed (p < 0.05). Moreover, because sperm suitable for oocyte fertilization is released from oviduct epithelial cells (OECs), in response to the capacitation process, we established an in vitro OEC culture model to select a sperm population with potential fertilizing capacity in this species. We demonstrated E-cadherin and cytokeratin expression in cultures of OECs obtained. When sperm–OEC cocultures were performed, the attached spermatozoa were motile and presented an intact acrosome, suggesting a selection by the oviductal model. When co-cultures were incubated in capacitating conditions a greater number of alive (p < 0.05), capacitated (p < 0.05), with progressive motility (p < 0.05) and with the intact acrosome sperm population was observed (p < 0.05) suggesting that the sperm population released from OECs in vitro presents potential fertilizing capacity. Improvements in handling and selection of cryopreserved sperm would improve efficiencies in ARTs allowing the use of a population of higher-quality sperm.
Palabras clave: ARTS , CRYOPRESERVED SPERM , EQUINES , SPERM SELECTION , SPERM–OVIDUCT INTERACTION
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)
Identificadores
URI: http://hdl.handle.net/11336/184252
URL: https://www.mdpi.com/2076-2615/11/1/74/htm
DOI: http://dx.doi.org//10.3390/ani11010074
Colecciones
Articulos (IIBIO)
Articulos de INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Articulos(INIGEM)
Articulos de INSTITUTO DE INMUNOLOGIA, GENETICA Y METABOLISMO
Citación
Gimeno, Brenda Florencia; Bariani, Maria Victoria; Laiz Quiroga, Lucía Belén; Martínez León, Eduardo Antonio; Von Meyeren, Micaela; et al.; Effects of in vitro interactions of oviduct epithelial cells with frozen–thawed stallion spermatozoa on their motility, viability and capacitation status; Multidisciplinary Digital Publishing Institute; Animals; 11; 1; 1-2021; 1-18
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