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dc.contributor.author
Kaur, Jaspreet
dc.contributor.author
Mazzone, Graciela Luján
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dc.contributor.author
Aquino, Jorge Benjamin
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dc.contributor.author
Nistri, Andrea
dc.date.available
2023-01-11T09:49:46Z
dc.date.issued
2021-09
dc.identifier.citation
Kaur, Jaspreet; Mazzone, Graciela Luján; Aquino, Jorge Benjamin; Nistri, Andrea; Nicotine Neurotoxicity Involves Low Wnt1 Signaling in Spinal Locomotor Networks of the Postnatal Rodent Spinal Cord; MDPI; International Journal of Molecular Sciences; 22; 17; 9-2021; 1-21
dc.identifier.issn
1422-0067
dc.identifier.uri
http://hdl.handle.net/11336/184234
dc.description.abstract
The postnatal rodent spinal cord in-vitro is a useful model to investigate early pathophysi-ological changes after injury. While low dose nicotine (1 µM) induces neuroprotection, how higher doses affect spinal networks is unknown. Using spinal preparations of postnatal wild-type Wistar rat and Wnt1Cre2:Rosa26Tom double-transgenic mouse, we studied the effect of nicotine (0.5–10 µM) on locomotor networks in-vitro. Nicotine 10 µM induced motoneuron depolarization, suppressed monosynaptic reflexes, and decreased fictive locomotion in rat spinal cord. Delayed fall in neuronal numbers (including motoneurons) of central and ventral regions emerged without loss of dorsal neurons. Conversely, nicotine (0.5–1 µM) preserved neurons throughout the spinal cord and strongly activated the Wnt1 signaling pathway. High-dose nicotine enhanced expression of S100 and GFAP in astrocytes indicating a stress response. Excitotoxicity induced by kainate was contrasted by nicotine (10 µM) in the dorsal area and persisted in central and ventral regions with no change in basal Wnt signaling. When combining nicotine with kainate, the activation of Wnt1 was reduced compared to kainate/sham. The present results suggest that high dose nicotine was neurotoxic to central and ventral spinal neurons as the neuroprotective role of Wnt signaling became attenuated. This also corroborates the risk of cigarette smoking for the foetus/newborn since tobacco contains nicotine.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
EXCITOTOXICITY
dc.subject
FICTIVE LOCOMOTION
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LOCOMOTOR NETWORKS
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MICE
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NICOTINE TOXICITY
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POSTNATAL
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RAT
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SPINAL CORD INJURY
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WNT1 PATHWAY
dc.subject.classification
Neurociencias
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Nicotine Neurotoxicity Involves Low Wnt1 Signaling in Spinal Locomotor Networks of the Postnatal Rodent Spinal Cord
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-21T14:10:48Z
dc.journal.volume
22
dc.journal.number
17
dc.journal.pagination
1-21
dc.journal.pais
Suiza
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dc.description.fil
Fil: Kaur, Jaspreet. Universidad de Copenhagen; Dinamarca
dc.description.fil
Fil: Mazzone, Graciela Luján. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
dc.description.fil
Fil: Aquino, Jorge Benjamin. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
dc.description.fil
Fil: Nistri, Andrea. International School For Advanced Studies; Italia
dc.journal.title
International Journal of Molecular Sciences
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/17/9572
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/ijms22179572
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