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dc.contributor.author
Adachi, Osao
dc.contributor.author
Hours, Roque Alberto
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dc.contributor.author
Shinagawa, Emiko
dc.contributor.author
Akakabe, Yoshihiko
dc.contributor.author
Yakushi, Toshiharu
dc.contributor.author
Matsushita, Kazunobu
dc.date.available
2023-01-10T13:34:51Z
dc.date.issued
2011-09
dc.identifier.citation
Adachi, Osao; Hours, Roque Alberto; Shinagawa, Emiko; Akakabe, Yoshihiko; Yakushi, Toshiharu; et al.; Formation of 4-keto-D-aldopentoses and 4-pentulosonates (4-keto-D-pentonates) with unidentified membrane-bound enzymes from acetic acid bacteria; Japan Soc Biosci Biotechn Agrochem; Bioscience, Biotechnology and Biochemistry; 75; 9; 9-2011; 1801-1806
dc.identifier.issn
0916-8451
dc.identifier.uri
http://hdl.handle.net/11336/184139
dc.description.abstract
In our previous study, a new microbial reaction yielding 4-keto-D-arabonate from 2,5-diketo-D-gluconate was identified with Gluconacetobacter liquefaciens RCTMR 10. It appeared that decarboxylation and dehydrogenation took place together in the reaction. To analyze the nature of the reaction, investigations were done with the membrane fraction of the organism, and 4-keto-D-arabinose was confirmed as the direct precursor of 4-keto-D-arabonate. Two novel membrane-bound enzymes, 2,5-diketo-D-gluconate decarboxylase and 4-keto-D-aldopentose 1-dehydrogenase, were involved in the reaction. Alternatively, D-arabonate was oxidized to 4-keto-D-arabonate by another membrane-bound enzyme, D-arabonate 4-dehydrogenase. More directly, D-arabinose oxidation was examined with growing cells and with the membrane fraction of G. suboxydans IFO 12528. 4-Keto-D-arabinose, the same intermediate as that from 2,5-diketo-D-gluconate, was detected, and it was oxidized to 4-keto-D-arabonate. Likewise, D-ribose was oxidized to 4-keto-D-ribose and then it was oxidized to 4-keto-D-ribonate. In addition to 4-keto-D-aldopentose 1-dehydrogenase, the presence of a novel membranebound enzyme, D-aldopentose 4-dehydrogenase, was confirmed in the membrane fraction. The formation of 4-keto-D-aldopentoses and 4-keto-D-pentonates (4-pentulosonates) was finally confirmed as reaction products of four different novel membrane-bound enzymes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Japan Soc Biosci Biotechn Agrochem
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
2,5-DIKETO-D-GLUCONATE DECARBOXYLASE
dc.subject
4-KETO-D-ALDOPENTOSE
dc.subject
4-KETO-DALDOPENTOSE 1-DEHYDROGENASE
dc.subject
4-KETO-DPENTONATE
dc.subject
D-ALDOPENTOSE 4-DEHYDROGENASE
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Formation of 4-keto-D-aldopentoses and 4-pentulosonates (4-keto-D-pentonates) with unidentified membrane-bound enzymes from acetic acid bacteria
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-04-23T19:09:51Z
dc.journal.volume
75
dc.journal.number
9
dc.journal.pagination
1801-1806
dc.journal.pais
Japón
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dc.journal.ciudad
Tokyo
dc.description.fil
Fil: Adachi, Osao. Yamaguchi University; Japón
dc.description.fil
Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.description.fil
Fil: Shinagawa, Emiko. Ube National College Of Technology; Japón
dc.description.fil
Fil: Akakabe, Yoshihiko. Yamaguchi University; Japón
dc.description.fil
Fil: Yakushi, Toshiharu. Yamaguchi University; Japón
dc.description.fil
Fil: Matsushita, Kazunobu. Yamaguchi University; Japón
dc.journal.title
Bioscience, Biotechnology and Biochemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1271/bbb.110339
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org//10.1271/bbb.110339
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