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dc.contributor.author
Eliades, Lorena Alejandra
dc.contributor.author
Rojas, Natalia Lorena
dc.contributor.author
Cabello, Marta Noemí
dc.contributor.author
Voget, Claudio Enrique
dc.contributor.author
Saparrat, Mario Carlos Nazareno
dc.date.available
2023-01-10T11:32:26Z
dc.date.issued
2011-12
dc.identifier.citation
Eliades, Lorena Alejandra; Rojas, Natalia Lorena; Cabello, Marta Noemí; Voget, Claudio Enrique; Saparrat, Mario Carlos Nazareno; α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis; Wiley VCH Verlag; Journal of Basic Microbiology; 51; 6; 12-2011; 659-665
dc.identifier.issn
0233-111X
dc.identifier.uri
http://hdl.handle.net/11336/184062
dc.description.abstract
α -L-Rhamnosidases (EC 3.2.1.40) and β -D-glucosidases (EC 3.2.1.21) obtained from several microbial sources are potential catalysts in food, beverage, and pharmaceutical industries. However, the enzyme preparations currently used have limitations related to the stability and activity of the enzyme as well to their reuse. A microtiter screening was carried out in 55 fungal strains isolated from alkaline soils, to obtain active α -L-rhamnosidases and β -D-glucosidases at pH 9.0. While α -L-rhamnosidase activity was detected in 45% of the strains tested, β -D-glucosidase activity was found only in 27%. Based on the fungal ability to produce α -L-rhamnosidase activity, cultures were supplemented with naringin to study the activities of the enzymes and the potential of the fungal strains on naringin hydrolysis. About 70% of the fungal strains tested increased the activities of both enzymes in the naringin-supplemented cultures as compared to non-supplemented ones. This effect was higher in Acrostalagmus luteo-albus LPSC 427 (15.3 fold) for α -L-rhamnosidase activity and Metarrhizium anisopliae LPSC 996 (51.1 fold) for β -D-glucosidase activity. All the enzyme preparations tested hydrolyzed naringin at pH 9.0, being that obtained from Acremonium murorun LPSC 927 cultures the one which showed highest hydrolysis. Here, different fungal species are reported for the first time for their ability to produce α -L-rhamnosidase and β -D-glucosidase activity at alkaline pH.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley VCH Verlag
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Α -L-RHAMNOSIDASE
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Β -D-GLUCOSIDASE
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ALKALINE ENZYMES
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FUNGI
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NARINGIN HYDROLYSIS
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Otras Ciencias Naturales y Exactas
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Otras Ciencias Naturales y Exactas
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CIENCIAS NATURALES Y EXACTAS
dc.title
α -L-Rhamnosidase and β -D-glucosidase activities in fungal strains isolated from alkaline soils and their potential in naringin hydrolysis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-04-23T19:09:43Z
dc.journal.volume
51
dc.journal.number
6
dc.journal.pagination
659-665
dc.journal.pais
Alemania
dc.journal.ciudad
Weinheim
dc.description.fil
Fil: Eliades, Lorena Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Rojas, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.description.fil
Fil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
dc.description.fil
Fil: Voget, Claudio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.description.fil
Fil: Saparrat, Mario Carlos Nazareno. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
dc.journal.title
Journal of Basic Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/jobm.201100163
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jobm.201100163
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