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Artículo

Optimizing culture medium composition to improve oligodendrocyte progenitor cell yields in vitro from subventricularzone-derived neural progenitor cell neurospheres

Franco, Paula GabrielaIcon ; Pasquini, Juana MariaIcon ; Silvestroff, LucasIcon
Fecha de publicación: 04/2015
Editorial: Public Library of Science
Revista: Plos One
ISSN: 1932-6203
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Neural Stem and Progenitor Cells (NSC/NPC) are gathering tangible recognition for their uses in cell therapy and cell replacement therapies for human disease, as well as a model system to continue research on overall neural developmental processes in vitro. The Subventricular Zone is one of the largest NSC/NPC niches in the developing mammalian Central Nervous System, and persists through to adulthood. Oligodendrocyte progenitor cell (OPC) enriched cultures are usefull tools for in vitro studies as well as for cell replacement therapies for treating demyelination diseases. We used Subventricular Zone-derived NSC/NPC primary cultures from newborn mice and compared the effects of different growth factor combinations on cell proliferation and OPC yield. The Platelet Derived Growth Factor-AA and BB homodimers had a positive and significant impact on OPC generation. Furthermore, heparin addition to the culture media contributed to further increase overall culture yields. The OPC generated by this protocol were able to mature into Myelin Basic Protein-expressing cells and to interact with neurons in an in vitro co-culture system. As a whole, we describe an optimized in vitro method for increasing OPC.
Palabras clave: Progenitor , Pdgf , Stem , Neuroscience
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/18372
DOI: http://dx.doi.org/10.1371/journal.pone.0121774
URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383518/
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Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Franco, Paula Gabriela; Pasquini, Juana Maria; Silvestroff, Lucas; Optimizing culture medium composition to improve oligodendrocyte progenitor cell yields in vitro from subventricularzone-derived neural progenitor cell neurospheres; Public Library of Science; Plos One; 10; 4; 4-2015; 1-21; e0121774
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