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dc.contributor.author
Regiart, Daniel Matias Gaston
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dc.contributor.author
Ledo, Ana
dc.contributor.author
Fernandes, Eliana
dc.contributor.author
Messina, Germán Alejandro
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dc.contributor.author
Brett, Christopher M. A.
dc.contributor.author
Bertotti, Mauro
dc.contributor.author
Barbosa, Rui M.
dc.date.available
2023-01-03T17:47:19Z
dc.date.issued
2022-03
dc.identifier.citation
Regiart, Daniel Matias Gaston; Ledo, Ana; Fernandes, Eliana; Messina, Germán Alejandro; Brett, Christopher M. A.; et al.; Highly sensitive and selective nanostructured microbiosensors for glucose and lactate simultaneous measurements in blood serum and in vivo in brain tissue; Elsevier Advanced Technology; Biosensors & Bioelectronics; 199; 3-2022; 1-10
dc.identifier.issn
0956-5663
dc.identifier.uri
http://hdl.handle.net/11336/183206
dc.description.abstract
Highly sensitive and selective nanostructured lactate and glucose microbiosensors for their in vivo simultaneous determination in rat brain were developed based on carbon fiber microelectrodes (CFM) modified with nanoporous gold (NPG) using the Dynamic Hydrogen Bubble Template (DHBT) method. Electrodeposition of platinum nanoparticles (PtNP) onto the NPG film enhances the sensitivity and the electrocatalytic properties towards H2O2 detection. The nanostructured microelectrode platform was modified by glucose oxidase (GOx) and lactate oxidase (LOx) enzyme immobilization. High selective measurements were achieved by covering with a perm-selective layer of electropolymerized m-phenylenediamine, deposition of a Nafion® film and by using a null sensor. The morphological characteristics and electroanalytical performance of the microbiosensors were assessed, by scanning electron microscopy and electrochemical techniques, respectively. The PtNP/NPG/CFM shows a high sensitivity to H2O2 (5.96 A M-1 cm-2) at 0.36 V vs. Ag/AgCl, with a linear range from 0.2 to 200 μM, and an LOD of 10 nM. The microbiosensors were applied to the simultaneous determination of lactate and glucose in blood serum samples. Moreover, the basal extracellular concentrations of lactate and glucose were measured in vivo in four different rat brain structures. These results support the potential of the microbiosensor to be used as a valuable tool to investigate brain neurochemicals in vivo.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Advanced Technology
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
GLUCOSE OXIDASE
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LACTATE OXIDASE
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MICROBIOSENSOR
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NANOPOROUS GOLD
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NANOSTRUCTURED
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PLATINUM NANOPARTICLES
dc.subject.classification
Química Analítica
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Highly sensitive and selective nanostructured microbiosensors for glucose and lactate simultaneous measurements in blood serum and in vivo in brain tissue
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-07-04T20:17:04Z
dc.journal.volume
199
dc.journal.pagination
1-10
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Regiart, Daniel Matias Gaston. Universidade de Sao Paulo; Brasil. Universidad de Coimbra; Portugal
dc.description.fil
Fil: Ledo, Ana. Universidad de Coimbra; Portugal
dc.description.fil
Fil: Fernandes, Eliana. Universidad de Coimbra; Portugal
dc.description.fil
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
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Fil: Brett, Christopher M. A.. Universidad de Coimbra; Portugal
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Fil: Bertotti, Mauro. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Barbosa, Rui M.. Universidad de Coimbra; Portugal
dc.journal.title
Biosensors & Bioelectronics
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bios.2021.113874
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